A Calmodulin-binding/CGCG Box DNA-binding Protein Family Involved in Multiple Signaling Pathways in Plants* 210
A Calmodulin-binding/CGCG Box DNA-binding Protein Family Involved in Multiple Signaling Pathways in Plants* 210
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DOI：
10.1074/jbc.m207941200
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发表时间：
2002-11
期刊：
影响因子：
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通讯作者：
Tianbao Yang;B. Poovaiah
中科院分区：
文献类型：
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作者：
Tianbao Yang;B. Poovaiah
我们早些时候报道了烟草早期乙烯反应基因NtER1编码一个钙调蛋白结合蛋白(Yang，T.和Poovaiah，B.W.(2000)J.Biol)。化学。275、38467-38473)。在这里，我们证明了在拟南芥中有一个NtER1同源基因和五个相关基因。环境信号如极端温度、UVB、盐和伤害；激素如乙烯和脱落酸；以及信号分子如茉莉酸甲酯、过氧化氢和水杨酸可以快速和差异地诱导这六个基因。因此，它们被命名为AtSR1-6(一个狂犬病信号反应基因)。Ca~(2+)/钙调蛋白与所有α结合，其钙调蛋白结合区位于C末端保守的两亲性ATSR-螺旋基序上。AtSR1以细胞核为靶标，特异性识别一种新型的6-碱基CGCG盒(A/C/G)CGCG(G/T/C)。在乙烯信号、脱落酸信号和光信号感受等基因的启动子中发现了多种CGCG顺式元件。AtSR1的DNA结合区位于N端146bp，所有与AtSR1相关的蛋白都有很高的相似性，但与其他已知的DNA结合蛋白没有相似性。从受伤叶片中分离的钙调素结合核蛋白具有特异性的CGCG盒DNA结合活性。这些结果表明，AtSR基因家族编码一个钙调蛋白/DNA结合蛋白家族，参与植物多种信号转导途径。
We reported earlier that the tobacco early ethylene-responsive gene NtER1 encodes a calmodulin-binding protein (Yang, T., and Poovaiah, B. W. (2000) J. Biol. Chem. 275, 38467–38473). Here we demonstrate that there is oneNtER1 homolog as well as five related genes inArabidopsis. These six genes are rapidly and differentially induced by environmental signals such as temperature extremes, UVB, salt, and wounding; hormones such as ethylene and abscisic acid; and signal molecules such as methyl jasmonate, H2O2, and salicylic acid. Hence, they were designated as AtSR1–6 ( A rabidopsisthaliana signal-responsive genes). Ca2+/calmodulin binds to all AtSRs, and their calmodulin-binding regions are located on a conserved basic amphiphilic α-helical motif in the C terminus. AtSR1 targets the nucleus and specifically recognizes a novel 6-bp CGCG box (A/C/G)CGCG(G/T/C). The multiple CGCG cis-elements are found in promoters of genes such as those involved in ethylene signaling, abscisic acid signaling, and light signal perception. The DNA-binding domain in AtSR1 is located on the N-terminal 146 bp where all AtSR1-related proteins share high similarity but have no similarity to other known DNA-binding proteins. The calmodulin-binding nuclear proteins isolated from wounded leaves exhibit specific CGCG box DNA binding activities. These results suggest that the AtSR gene family encodes a family of calmodulin-binding/DNA-binding proteins involved in multiple signal transduction pathways in plants.