Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
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DOI：
10.1098/rsos.200027
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发表时间：
2020-07
影响因子：
3.5
通讯作者：
S. Yamahira;T. Satoh;F. Yanagawa;M. Tamura;T. Takagi;Eri Nakatani;Yuta Kusama;K. Sumaru;S. Sugiura;T. Kanamori
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文献类型：
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作者：
S. Yamahira;T. Satoh;F. Yanagawa;M. Tamura;T. Takagi;Eri Nakatani;Yuta Kusama;K. Sumaru;S. Sugiura;T. Kanamori
在生物体内，浓度梯度随着生物过程的进展而动态变化。因此，需要以空间控制的方式构建动态微尺度浓度梯度的方法来提供更真实的研究环境。在这里，我们报告了一种新的方法，用于在微图案化水凝胶中以逐步的方式在细胞周围构建动态微尺度浓度梯度。在我们的方法中，细胞被封装在微流体灌注培养装置内形成的可光降解水凝胶中，然后通过微图案化光降解在水凝胶中制造灌注微通道。然后可以通过将培养基灌注通过制造的微通道来培养微图案化水凝胶中的细胞。通过使用这种方法，我们展示了两个动态浓度梯度的同时构建，这使我们能够将封装在水凝胶中的细胞暴露于动态微环境中。
Inside living organisms, concentration gradients dynamically change over time as biological processes progress. Therefore, methods to construct dynamic microscale concentration gradients in a spatially controlled manner are needed to provide more realistic research environments. Here, we report a novel method for the construction of dynamic microscale concentration gradients in a stepwise manner around cells in micropatterned hydrogel. In our method, cells are encapsulated in a photodegradable hydrogel formed inside a microfluidic perfusion culture device, and perfusion microchannels are then fabricated in the hydrogel by micropatterned photodegradation. The cells in the micropatterned hydrogel can then be cultured by perfusing culture medium through the fabricated microchannels. By using this method, we demonstrate the simultaneous construction of two dynamic concentration gradients, which allowed us to expose the cells encapsulated in the hydrogel to a dynamic microenvironment.