CALCIUM TRANSIENTS EVOKED BY CLIMBING FIBER AND PARALLEL FIBER SYNAPTIC INPUTS IN GUINEA-PIG CEREBELLAR PURKINJE NEURONS
CALCIUM TRANSIENTS EVOKED BY CLIMBING FIBER AND PARALLEL FIBER SYNAPTIC INPUTS IN GUINEA-PIG CEREBELLAR PURKINJE NEURONS
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DOI：
10.1152/jn.1992.68.4.1178
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发表时间：
1992-10-01
影响因子：
2.5
通讯作者：
ROSS, WN
中科院分区：
文献类型：
--
作者：
MIYAKAWA, H;LEVRAM, V;ROSS, WN
1.在豚鼠小脑脑片浦肯野细胞上记录了与爬行纤维（CF）和平行纤维（PF）突触电位相关的钙瞬变。使用偶氮胂III的吸光度变化或fura-2的荧光变化测量瞬态，将其注射到切片中的单个细胞中。由白色物质刺激引起的全部或无体细胞记录的CF电位具有全部或无Ca瞬变。这些信号从电记录的突触电位的开始以大于或等于2 ms的延迟开始。CF诱导的偶氮胂III吸光度瞬变的恢复时间为
1. Calcium transients related to climbing fiber (CF) and parallel fiber (PF) synaptic potentials were recorded from Purkinje cells in guinea pig cerebellar slices. Transients were measured using either absorbance changes of arsenazo III or fluorescence changes of fura-2, which were injected into individual cells in the slice.2. All-or-none somatically recorded CF potentials elicited by white matter stimulation had all-or-none Ca transients. These signals began with a delay of greater-than-or-equal-to 2 ms from the start of the electrically recorded synaptic potential. The recovery time of CF-induced arsenazo III absorbance transients was