Assimilation efficiency of Vibrio bacterial protein biomass by the flagellate Pteridomonas : assessment using flow cytometric sorting.
Assimilation efficiency of Vibrio bacterial protein biomass by the flagellate Pteridomonas : assessment using flow cytometric sorting.
复制标题
鞭毛蕨单胞菌对弧菌细菌蛋白质生物量的同化效率：使用流式细胞术分选进行评估。
DOI：
10.1016/j.femsec.2005.04.001
复制
发表时间：
2005
影响因子：
4.2
通讯作者：
M. Sleigh
中科院分区：
文献类型：
--
作者：
M. Zubkov;M. Sleigh
开发了一种流式细胞术分选技术，用于直接测定吞噬鞭毛虫对细菌生物量的同化作用，并在实验室培养实验中进行了测试。用[35S]甲硫氨酸示踪剂对活的弧菌进行定量脉冲追踪标记，并将其喂给翼虫鞭毛虫。流式分选显示，同位素标记的材料位于细菌猎物或鞭毛捕食者中，并且排出的细菌碎片含有可忽略不计的示踪剂。这些实验结果证实了早期的假设，即鞭毛虫主要以溶解的形式释放代谢的细菌蛋白。蕨藻对弧菌蛋白生物量的同化效率很低，仅为 20% 左右，与消耗的细菌生物量无关，证实了我们之前的间接估计。此外，与细胞在准备和参与分裂时减少代谢活动的预期相反，我们发现流式分选细菌细胞在S + G2细胞周期阶段的前体摄取率始终比G1阶段细胞高1.5倍。
A flow cytometric sorting technique for direct determination of bacterial biomass assimilation by phagotrophic flagellates was developed and tested in laboratory culture experiments. LivingVibriobacteria were quantitatively pulse-chase labelled with [35S]methionine tracer and fed toPteridomonasflagellates. Flow sorting revealed that the isotopically labelled material is in either bacterial prey or flagellate predators and the egested bacterial debris contained negligible amounts of tracer. These experimental results confirm an earlier hypothesis that flagellates release metabolised bacterial proteins primarily in a dissolved form. The assimilation efficiency of theVibrioprotein biomass byPteridomonaswas low, only about 20%, independently of the amount of consumed bacterial biomass, confirming our earlier indirect estimates. Additionally, against expectations that cells decrease their metabolic activity whilst preparing for and engaged in division, we found that the precursor uptake rates by flow sorted bacterial cells at the S + G2cell cycle stages were constantly 1.5 times higher than those of cells at the G1stage.