Effect of locally applied drugs on the pH of luminal fluid in the endolymphatic sac of guinea pig
Effect of locally applied drugs on the pH of luminal fluid in the endolymphatic sac of guinea pig
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DOI：
10.1152/ajpregu.2000.279.5.r1695
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发表时间：
2000-11-01
影响因子：
2.8
通讯作者：
Ferrary, E
中科院分区：
文献类型：
--
作者：
Couloigner, V;Teixeira, M;Ferrary, E
本工作的目的是评估局部应用的各种药物对豚鼠内淋巴囊腔液pH值（pH（lum））的影响。当通过双管pH敏感微电极在体内测量时，pH（lum）和跨上皮电位分别为7.06 +/- 0.08和+6.1 +/- 0.34 mV（平均值+/- SE; n = 84），这与内淋巴囊腔液中的净酸分泌一致。巴弗洛霉素和乙酰唑胺分别升高和降低pH（lum）。阿米洛利、乙基异丙基阿米洛利、哇巴因和Schering 28080对pH（lum）无影响。用阴离子转运系统抑制剂获得的结果是不确定的;例如，DIDS降低pH（lum），而SITS和triflocin均无任何影响。我们的结论是，巴非霉素敏感的H+-ATP酶活性占跨上皮内淋巴囊中测得的酸梯度和细胞内和膜结合的碳酸酐酶可能参与调节内淋巴囊pH值（流明）。酸性pH、内淋巴体积与梅尼埃病之间的关系有待进一步研究。
The aim of the present work was to assess the effect of various drugs applied locally on the pH of the luminal fluid (pH(lum)) in guinea pig endolymphatic sac. pH(lum) and transepithelial potential, when measured in vivo by means of double-barrelled pH-sensitive microelectrodes, were 7.06 +/- 0.08 and +6.1 +/- 0.34 mV (mean +/- SE; n = 84), respectively, which is consistent with a net acid secretion in the luminal fluid of the endolymphatic sac. Bafilomycin and acetazolamide increased and decreased, respectively, pH(lum). Amiloride, ethylisopropylamiloride, ouabain, and Schering 28080 had no effect on pH(lum). Results obtained with inhibitors of anionic transport systems were inconclusive; e.g., DIDS reduced pH(lum), whereas neither SITS nor triflocin had any effect. We conclude that bafilomycin-sensitive H+-ATPase activity accounts for the transepithelial acid gradient measured in the endolymphatic sac and that intracellular and membrane-bound carbonic anhydrase probably participates in regulating endolymphatic sac pH(lum). The relationship between acid pH, endolymph volume, and Meniere's disease remains to be further investigated.