FREQUENT ISOLATION OF HUMAN HERPESVIRUS-7 FROM SALIVA
FREQUENT ISOLATION OF HUMAN HERPESVIRUS-7 FROM SALIVA
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从唾液中频繁分离出人类疱疹病毒-7
DOI：
10.1016/0168-1702(93)90128-a
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发表时间：
1993-07-01
期刊：
影响因子：
5
通讯作者：
PELLETT, PE
中科院分区：
文献类型：
--
作者：
BLACK, JB;INOUE, N;PELLETT, PE
用人脐带血淋巴细胞培养唾液，从8例健康成人中的6例获得人疱疹病毒7型（HHV-7）分离株。根据限制性内切酶酶切图谱的比较及与HHV-7株RK DNA的杂交结果，将这些分离株鉴定为HHV-7。分离株与HHV-7 RK株的限制性内切酶图谱可以区分。我们证实了从健康成人唾液中频繁分离到HHV-7的发现，并报告了首次从单个唾液标本中分离到人类疱疹病毒6型（HHV-6）和HHV-7。我们还描述了一种原位杂交试验，可以区分HHV-6和HHV-7。
We obtained isolates of human herpesvirus 7 (HHV-7) from 6 of 8 healthy adults by culturing saliva with human umbilical cord blood lymphocytes. These isolates were identified as HHV-7 on the basis of comparisons of restriction endonuclease fragment profiles and hybridization with HHV-7 strain RK DNA. The isolates could be differentiated from HHV-7 strain RK and from each other by their restriction endonuclease fragment profiles. We confirm the finding of frequent isolation of HHV-7 from saliva of healthy adults and report the first dual isolation of human herpesvirus 6 (HHV-6) and HHV-7 from a single saliva specimen. We also describe an in situ hybridization assay that can distinguish between HHV-6 and HHV-7.