Electrospun nanofiber sheets incorporating methylcobalamin promote nerve regeneration and functional recovery in a rat sciatic nerve crush injury model
Electrospun nanofiber sheets incorporating methylcobalamin promote nerve regeneration and functional recovery in a rat sciatic nerve crush injury model
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DOI：
10.1016/j.actbio.2017.02.004
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发表时间：
2017-04-15
影响因子：
9.7
通讯作者：
Yoshikawa, Hideki
中科院分区：
文献类型：
--
作者：
Suzuki, Koji;Tanaka, Hiroyuki;Yoshikawa, Hideki
周围神经损伤是常见的创伤之一。虽然受损的周围神经有再生能力，但轴突再生进展缓慢，功能结果往往很差。药理学增强再生可以在促进功能恢复方面发挥重要作用。在这项研究中，我们开发了一种新的静电纺丝纤维板，将维生素B12同系物的活性形式之一甲钴胺（MeCbl），提供足够的局部周围神经损伤部位。我们评估了在神经损伤部位局部施用MeCbl是否有效促进神经再生。当在体外测试时，静电纺丝片材逐渐释放MeCbl至少8周。在外周神经系统的体内功能中，无不良反应。局部植入含MeCbl的复合片有助于坐骨神经损伤后运动和感觉功能的恢复，神经传导速度的恢复，以及促进髓鞘形成。不影响MeCbl的血浆浓度。显著性声明甲基钴胺素（MeCbl）是一种维生素B12类似物，我们以前报道过它在体外和体内对神经元轴突生长和雪旺细胞分化的有效性。vivo.在这里，我们估计了局部施用MeCbl与静电纺丝的纤维素膜对周围神经损伤的影响。局部给予MeCbl促进大鼠坐骨神经挤压损伤模型的功能恢复。这些片可用于连续性神经损伤，不同于人工神经导管，人工神经导管仅用于神经缺损。我们相信，这项研究的结果与您的期刊范围相关，并将引起读者的兴趣。(C)2017 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Peripheral nerve injury is one of common traumas. Although injured peripheral nerves have the capacity to regenerate, axon regeneration proceeds slowly and functional outcomes are often poor. Pharmacological enhancement of regeneration can play an important role in increasing functional recovery. In this study, we developed a novel electrospun nanofiber sheet incorporating methylcobalamin (MeCbl), one of the active forms of vitamin B12 homologues, to deliver it enough locally to the peripheral nerve injury site. We evaluated whether local administration of MeCbl at the nerve injury site was effective in promoting nerve regeneration. Electrospun nanofiber sheets gradually released MeCbl for at least 8 weeks when tested in vitro. There was no adverse effect of nanofiber sheets on function in vivo of the peripheral nervous system. Local implantation of nanofiber sheets incorporating MeCbl contributed to the recovery of the motor and sensory function, the recovery of nerve conduction velocity, and the promotion of myelination after sciatic nerve injury, without affecting plasma concentration of MeCbl.Statement of SignificanceMethylcobalamin (MeCbl) is a vitamin B12 analog and we previously reported its effectiveness in axonal outgrowth of neurons and differentiation of Schwann cells both in vitro and in vivo. Here we estimated the effect of local administered MeCbl with an electrospun nanofiber sheet on peripheral nerve injury. Local administration of MeCbl promoted functional recovery in a rat sciatic nerve crush injury model. These sheets are useful for nerve injury in continuity differently from artificial nerve conduits, which are useful only for nerve defects. We believe that the findings of this study are relevant to the scope of your journal and will be of interest to its readership. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.