Frequent loss of membranous E-cadherin in gastric cancers:: A cross-talk with Wnt in determining the fate of β-catenin
Frequent loss of membranous E-cadherin in gastric cancers:: A cross-talk with Wnt in determining the fate of β-catenin
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DOI：
10.1007/s10585-005-4578-8
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Li, H
中科院分区：
文献类型：
--
作者：
Cheng, XX;Wang, ZC;Li, H
采用基于冷冻组织阵列的免疫组化、Western blot和RT-PCR分析方法，探讨E-cadherin减少与Wnt2上调在胃癌细胞内β -catenin分布中的潜在相关性。结果显示，膜性E-cadherin在两种主要胃癌亚型（肠胃癌i-GC和弥漫性胃癌d-GC）中频繁降低，且与淋巴结转移风险密切相关（P < 0.05）。细胞膜E-cadherin的减少与细胞质和细胞核中β -catenin的积累以及Wnt2表达的增加是平行的。这些结果表明，e -钙粘蛋白的减少是GCs常见的遗传表型，并在肿瘤转移中发挥有益作用。β -catenin分布的改变可能是由于E-cadherin产生和Wnt表达的不平衡导致的，这使得胃癌细胞具有更强的攻击性行为。
The potential correlation of E-cadherin reduction and Wnt2 up-regulation in determining the intracellular distribution of beta-catenin in gastric cancers was investigated by the methods of frozen tissue array-based immunohistochemistry, Western blot and RT-PCR analysis. It was revealed that membranous E-cadherin was reduced frequently in the two major subtypes of gastric cancer (intestinal gastric cancer, i-GC and diffuse gastric cancer, d-GC) and closely correlated with the risk of lymphoid node metastasis (P < 0.05). The reduction of membranous E-cadherin was paralleled with cytosolic and nuclear accumulation of beta-catenin and the increased Wnt2 expression. These results indicate that the reduced E-cadherin is a common genetic phenotype of GCs and plays beneficial roles in tumor metastasis. Altered beta-catenin distribution may result from the imbalance of E-cadherin production and Wnt expression, which confers on gastric cancer cells more aggressive behaviors.