Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers
Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers
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DOI：
10.1111/j.1439-0523.2011.01944.x
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发表时间：
2012-04-01
期刊：
影响因子：
2
通讯作者：
Barth, Susanne
中科院分区：
文献类型：
--
作者：
Tomaszewski, Celine;Byrne, Stephen L.;Barth, Susanne
多年生黑麦草是温带农业的主要牧草。近年来，已经做出了重大努力，以开发分子标记策略，以允许同时对大量基因座进行成本有效的表征。其中一种策略涉及使用DArT标记，最近已经为Lolium-Festuca复合体开发了DArT阵列。在这项研究中，我们报告的第一次使用的DArTFest阵列生成的遗传连锁图谱的基础上，在多年生黑麦草F2群体，由325个基因型的326个标记。为了证明概念，该图谱用于鉴定与冠锈病易感性差异相关的QTL，所述冠锈病易感性差异是由真菌生养型冠柄锈菌（Puccinia coronata）引起的。
Perennial ryegrass is the principal forage grass species used in temperate agriculture. In recent years, significant efforts have been made to develop molecular marker strategies to allow cost-effective characterization of a large number of loci simultaneously. One such strategy involves using DArT markers, and a DArT array has recently been developed for the Lolium-Festuca complex. In this study, we report the first use of the DArTFest array to generate a genetic linkage map based on 326 markers in a Lolium perenne F2 population, consisting of 325 genotypes. For proof of concept, the map was used to identify QTL associated with differences in crown rust susceptibility, caused by the fungal biotroph, Puccinia coronata.