Characterization of a blue-copper protein, auracyanin, of the filamentous anoxygenic phototrophic bacterium Roseiflexus castenholzii.
Characterization of a blue-copper protein, auracyanin, of the filamentous anoxygenic phototrophic bacterium Roseiflexus castenholzii.
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DOI：
10.1016/j.abb.2009.08.003
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发表时间：
2009-10
影响因子：
3.9
通讯作者：
Y. Tsukatani;Nahomi Nakayama;K. Shimada;H. Mino;S. Itoh;K. Matsuura;S. Hanada;K. Nagashima
中科院分区：
文献类型：
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作者：
Y. Tsukatani;Nahomi Nakayama;K. Shimada;H. Mino;S. Itoh;K. Matsuura;S. Hanada;K. Nagashima
对丝状厌氧光养菌Roseiflexus castenholzii的蓝铜蛋白金花青素进行了纯化和鉴定。基因组序列分析表明，R. Castenholzii只有一种金花青苷，而已知Chloroflexus aurantiacus有两种金花青苷A和B。玫瑰弯折藻的吸收光谱与C.金鱼藻的金鱼藻素B相似。橙黄色另一方面，玫瑰弯藻的ESR谱与C.橙黄色这些结果表明，蓝铜蛋白金花青素从R。castenholzii与auracyanin A和B中的每一个具有相同的特征。来自丝状无氧光养生物的金花青苷的氨基酸序列比对也证明了Roseiflexus金花青苷的一级结构的嵌合特征，即，auracyanin A-样氨基末端特征和auracyanin B-样羧基末端Cu-结合环处的一个残基间隔。
A blue-copper protein auracyanin of the filamentous anoxygenic phototroph Roseiflexus castenholzii was purified and characterized. Genomic sequence analysis showed that R. castenholzii has only one auracyanin, whereas Chloroflexus aurantiacus is known to have two auracyanins, A and B. Absorption spectrum of the Roseiflexus auracyanin was similar to that of auracyanin B of C. aurantiacus. On the other hand, ESR spectrum of the Roseiflexus auracyanin resembles that of auracyanin A of C. aurantiacus. These results suggest that the blue-copper protein auracyanin from R. castenholzii shares features with each of auracyanin A and B. Amino acid sequence alignment of auracyanins from filamentous anoxygenic phototrophs also demonstrated the chimeral feature of the primary structure of the Roseiflexus auracyanin, i.e., auracyanin A-like amino-terminal characteristics and auracyanin B-like one-residue spacing at the Cu-binding loop in the carboxyl-terminus.