Inbred strain preference in the BTBR T+ Itpr3tf/J mouse model of autism spectrum disorder: Does the stranger mouse matter in social approach?
Inbred strain preference in the BTBR T+ Itpr3tf/J mouse model of autism spectrum disorder: Does the stranger mouse matter in social approach?
复制标题
自闭症谱系障碍 BTBR T Itpr3tf/J 小鼠模型中的近交系偏好：陌生人小鼠在社交方式中重要吗？
DOI：
10.1002/aur.2158
复制
发表时间：
2019
期刊：
影响因子：
4.7
通讯作者：
K. Chadman
中科院分区：
文献类型：
--
作者：
K. Ryan;Lynn Thompson;P. A. Mendoza;K. Chadman
BTBR T+ Itpr 3 tf/J（BTBR）小鼠由于其低水平的社交能力和高水平的重复梳理而被用作自闭症谱系障碍（ASD）的模型。这些实验使用三腔室社会接近测试的变体探索了BTBR和C57 BL/6 J小鼠的社会行为。在第一个测试中，受试小鼠在同一品系的陌生小鼠或具有不同社交水平的品系之间进行选择。BTBR雄性小鼠表现出对更社会化的C57 BL/6 J陌生小鼠的强烈偏好，C57 BL/6 J雄性小鼠也是如此，尽管仅在嗅闻时间上更温和。C57 BL/6 J雌性小鼠仅在嗅闻时间上对BTBR陌生小鼠表现出中度偏好，而BTBR雌性小鼠未表现出偏好。第二个实验检查受试小鼠是否更喜欢陌生小鼠或来自陌生小鼠笼的垫料。雄性BTBR小鼠总是喜欢床上用品，而C57 BL/6 J雄性小鼠没有表现出偏好。BTBR和C57 BL/6 J雌性小鼠都喜欢当陌生小鼠是一个不同的品系，但不是当陌生小鼠是同一品系的床上用品。因此，陌生小鼠品系似乎对雌性小鼠的偏好影响大于雄性小鼠。老鼠更喜欢在有社交气味的房间里呆着，而不是真正的陌生老鼠，尽管并不总是很明显。这表明与陌生老鼠接触比气味更能引起压力或焦虑。自闭症研究2019，12：1184-1191。© 2019国际自闭症研究学会，Wiley Periodicals，Inc.
BTBR T+ Itpr3tf/J (BTBR) mice have been used as a model of autism spectrum disorder (ASD) due to their low levels of sociability and high levels of repetitive grooming. These experiments explored social behavior in the BTBR and C57BL/6J mice using variations of the three‐chambered social approach test. In the first test, the subject mice had a choice between a stranger mouse of the same strain or from a strain with a different level of sociability. The BTBR male mice demonstrated a strong preference for the more social C57BL/6J stranger mouse, as did the C57BL/6J male mice, although more moderately with sniff time only. The C57BL/6J female mice showed a moderate preference, sniff time only, for the BTBR stranger mouse, whereas the BTBR female mice did not show a preference. The second experiment examined whether the subject mouse preferred a stranger mouse or bedding from the stranger mouse home cage. Male BTBR mice always preferred bedding, whereas the C57BL/6J male mice did not show a preference. Both BTBR and C57BL/6J female mice preferred bedding when the stranger mouse was a different strain but not when the stranger mouse was the same strain. Therefore, the stranger mouse strain seems to influence the preference of the female mice more than the male mice. The mice preferred spending time in the chamber with the social smell but not the actual stranger mouse although not always significantly. This suggests that contact with a stranger mouse is more stressful or anxiety provoking than the smell. Autism Res 2019, 12: 1184–1191. © 2019 International Society for Autism Research, Wiley Periodicals, Inc.