Identification of the carboxyl termini of porcine zona pellucida glycoproteins ZPB and ZPC.
Identification of the carboxyl termini of porcine zona pellucida glycoproteins ZPB and ZPC.
复制标题
猪透明带糖蛋白 ZPB 和 ZPC 羧基末端的鉴定。
DOI：
10.1016/s0006-291x(03)01297-x
复制
发表时间：
2003
影响因子：
3.1
通讯作者：
M. Nakano
中科院分区：
文献类型：
--
作者：
N. Yonezawa;M. Nakano
哺乳动物卵母细胞周围的细胞外基质在受精中发挥重要作用，被称为透明带 (ZP)。 ZP 由三种糖蛋白 ZPA、ZPB 和 ZPC 组成，它们包含称为 ZP 结构域的同源区域。 ZP 结构域也存在于许多其他分泌性糖蛋白中。当蛋白质通过分泌途径时，存在于 ZP 糖蛋白前体 C 末端的推定跨膜结构域被去除。然而，这一处理的细节尚不清楚。特别是，哺乳动物透明带蛋白 C 末端的精确位置尚未确定。在本研究中，通过对猪ZPB和ZPC的C端多肽片段进行质谱分析，将这些蛋白的C端残基分别鉴定为Ala-462和Ser-332。这些结果表明，ZPB 在其弗林蛋白酶共有位点处被加工，而 ZPC 在弗林蛋白酶共有位点的 N 末端被加工。此外，对猪ZPB和ZPC片段的分析表明，ZP结构域内的二硫键分为两组，表明ZP结构域由两个子结构域组成。
The extracellular matrix surrounding mammalian oocytes plays important roles in fertilization and is known as the zona pellucida (ZP). The ZP consists of three glycoproteins, ZPA, ZPB, and ZPC, which contain homologous regions known as ZP domains. The ZP domain is also found in many other secretory glycoproteins. Putative transmembrane domains present at the C-termini of ZP glycoprotein precursors are removed as the proteins proceed through the secretory pathway. However, the details of this processing have been unclear. In particular, the precise locations of the C-termini of mammalian zona proteins have not yet been determined. In this study, the C-terminal residues of porcine ZPB and ZPC were identified as Ala-462 and Ser-332, respectively, by mass spectrometry of C-terminal polypeptide fragments of these proteins. These results suggest that ZPB is processed at its furin consensus site, whereas ZPC is processed N-terminal to the furin consensus site. In addition, the analyses of porcine ZPB and ZPC fragments revealed that disulfide bonds within the ZP domains are divided into two groups, suggesting that the ZP domain consists of two subdomains.
影响因子：
3.3
作者：
H. Qi;Zev Williams;P. M. Wassarman
通讯作者：
H. Qi;Zev Williams;P. M. Wassarman