Crystal structure of the 2-iminoglutarate-bound complex of glutamate dehydrogenase from Corynebacterium glutamicum.
Crystal structure of the 2-iminoglutarate-bound complex of glutamate dehydrogenase from Corynebacterium glutamicum.
复制标题
谷氨酸棒杆菌谷氨酸脱氢酶 2-亚氨基戊二酸结合复合物的晶体结构。
DOI：
10.1002/1873-3468.12667
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发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Nishiyama M.
中科院分区：
文献类型：
--
作者：
Tomita T;Yin L;Nakamura S;Kosono S;Kuzuyama T;Nishiyama M.
谷氨酸棒状杆菌的NADP+依赖性谷氨酸脱氢酶（CgGDH）具有较高的谷氨酸生成活性，被认为是谷氨酸工业发酵的关键酶之一。我们测定了CgGDH与NADP+和2‐亚氨基葡萄糖酸盐络合的晶体结构。在六聚体CgGDH -结合NADP+的六个亚基中，只有四个亚基以封闭形式结合2 -亚氨基葡萄糖酸酯，而其他两个亚基以开放形式结合。在封闭形式下，2 -亚氨基戊二酸与辅酶的烟酰胺环堆积的2 -亚胺基结合在底物结合位点上，表明在与亚胺中间体的假设反应方案中存在预氢化转移状态。我们还进行了MD模拟，并提供了CgGDH对谷氨酸产生反应的极端偏好的见解。原子坐标和结构因子已存入RCSB PDB数据库，登录号为5GUD。
The NADP+‐dependent glutamate dehydrogenase fromCorynebacterium glutamicum(CgGDH) is considered to be one of the key enzymes in the industrial fermentation of glutamate due to its high glutamate‐producing activity. We determined the crystal structure of CgGDH complexed with NADP+and 2‐iminoglutarate. Among six subunits of hexameric CgGDH‐binding NADP+, only four subunits bind 2‐iminoglutarate in a closed form, while the other two are in an open form. In the closed form, 2‐iminoglutarate is bound to the substrate‐binding site with the 2‐imino group stacked by the nicotinamide ring of the coenzyme, suggesting a prehydride transfer state in a hypothesized reaction scheme with the imino intermediate. We also conducted MD simulations and provide insights into the extreme preference for the glutamate‐producing reaction of CgGDH.DatabaseThe atomic coordinate and structure factors have been deposited in the RCSB PDB database under the accession number 5GUD.