Evidence that the nonsense-mediated mRNA decay pathway participates in X chromosome dosage compensation in mammals
Evidence that the nonsense-mediated mRNA decay pathway participates in X chromosome dosage compensation in mammals
复制标题
无义介导的 mRNA 衰减途径参与哺乳动物 X 染色体剂量补偿的证据
DOI：
10.1016/j.bbrc.2009.04.021
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发表时间：
2009-06-05
影响因子：
3.1
通讯作者：
Kong, Xiangyin
中科院分区：
文献类型：
--
作者：
Yin, Shanye;Deng, Wenjun;Kong, Xiangyin
目前的X染色体剂量补偿模型通常是参照转录水平的调节如何提高活性X染色体的基因表达来构建的。然而，这个框架可能过于简单，因为基因表达的调控也可以在转录后水平起作用。在这里，经过全基因组调查，我们发现，常染色体基因更容易受到无义介导的mRNA衰变（NMD）比X连锁基因。此外，我们证明，在NMD抑制后，X染色体和常染色体之间的平衡基因表达被破坏，使得全局平均X/常染色体基因表达比率降低10- 15%。我们的研究结果确定NMD作为一个转录后水平的调节机制，有助于观察到的微调哺乳动物的X染色体剂量补偿。(C)2009 Elsevier Inc. All rights reserved.
Current models of X chromosome dosage compensation are usually framed by reference to how regulation in transcriptional level elevates the gene expression of the active X chromosome. This framework, however, might be oversimplified because regulation of gene expression can also act at the post-transcriptional level. Here, after a genome-wide survey, we find that autosomal genes are more likely subject to nonsense-mediated mRNA decay (NMD) than X-linked genes. Furthermore, we demonstrate that after NMD inhibition, balanced gene expression between X chromosome and autosomes is corrupted such that the global mean X/autosome gene expression ratio is decreased by 10-15%. Our results identify NMD as a post-transcription-level regulatory mechanism that contributes to the observed fine-tuning of X chromosome dosage compensation in mammals. (C) 2009 Elsevier Inc. All rights reserved.