URINARY-EXCRETION OF PODOCALYXIN INDICATES GLOMERULAR EPITHELIAL-CELL INJURIES IN GLOMERULONEPHRITIS
URINARY-EXCRETION OF PODOCALYXIN INDICATES GLOMERULAR EPITHELIAL-CELL INJURIES IN GLOMERULONEPHRITIS
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DOI：
10.1159/000188509
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发表时间：
1995-04-01
期刊：
影响因子：
2.5
通讯作者：
KIHARA, I
中科院分区：
文献类型：
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作者：
HARA, M;YAMAMOTO, T;KIHARA, I
尿沉淀物的免疫荧光研究进行检测肾小球上皮细胞损伤，使用特异性单克隆抗体对足糖萼蛋白，一种糖蛋白，是显着表达的肾小球上皮细胞。各种肾小球疾病的尿沉渣中可见到管型、颗粒和细胞三种结构。尿中足糖萼蛋白的存在通过吸收试验、免疫电镜和蛋白质印迹证实。足糖萼蛋白在各种肾小球肾炎患者的尿沉渣中均有检出，尤其是急性起病的肾小球肾炎患者，其尿排泄量的多少明显反映了肾小球疾病时肾小球上皮细胞损伤的程度。
Immunofluorescent study of urine sediments was performed to detect glomerular epithelial cell injuries using specific monoclonal antibody against podocalyxin, a glycoprotein that is prominently expressed on glomerular epithelial cells. Three kinds of structures (casts, granules and cells) were stained in urine sediments from various glomerular diseases. The presence of podocalyxin in urines was confirmed by absorption test, immunoelectron microscopy and Western blotting. Podocalyxin was detected in the urinary sediments of patients with various forms of glomerulonephritis, particularly those with acute onset, The amount of their urinary excretion apparently indicates the degree of the glomerular epithelial cell injuries in glomerular diseases.