AMMONIA AND HYDROGEN-PRODUCTION BY IMMOBILIZED CYANOBACTERIA
AMMONIA AND HYDROGEN-PRODUCTION BY IMMOBILIZED CYANOBACTERIA
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DOI：
10.1016/0168-1656(86)90012-x
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发表时间：
1986-04-01
影响因子：
4.1
通讯作者：
HALL, DO
中科院分区：
文献类型：
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作者：
BROUERS, M;HALL, DO
将异囊蓝藻满江红鱼腥藻（共生）和Mastigocladus laminosus（嗜热）固定在聚乙烯或聚氨酯泡沫和藻酸盐中。固定后，当在合适的生长培养基中在光照下孵育时，基质逐渐定植。聚乙烯和聚氨酯泡沫固定化细胞的扫描电子显微镜研究表明，连接到基质表面的丝状粘液的发展。固定化导致增加和/或稳定的H2光生产率。这种增加主要是通过氢化酶介导的生产。固定化也稳定了固氮酶活性，并增加了两种蓝藻固氮的初始速率。在谷氨酰胺合成酶活性抑制剂L-甲硫氨酸-D，L-亚砜亚胺存在下监测氨的光产生。在间歇式反应器中从聚乙烯泡沫固定的A. azollae和M. laminosus，而对照自由生活培养物在相同条件下产生少于10 μ mol氨。当海藻酸固定化A.丙酮预处理增加了满江红。这表明在聚乙烯泡沫中固定化的氨产生的增加至少部分地与由固定化过程本身作为细胞-基质相互作用的结果诱导的膜渗透性的变化有关。
Heterocystous cyanobacteria Anabaena azollae (symbiotic) and Mastigocladus laminosus (thermophilic) were immobilized in polyvinyl or polyurethane foams and in alginate. Following immobilization there was a progressive colonization of the matrices when incubated under light in suitable growth media. Scanning electron microscopy studies of polyvinyl and polyurethane foam immobilized cells showed the development of a filamentous mucilage connected to the surface of the matrix. Immobilization led to an increase and/or to a stabilization of the rate of H2 photoproduction. This increase occurred mainly via hydrogenase-mediated production. Immobilization also stabilized nitrogenase activity and increased initial rates of nitrogen fixation by both species of cyanobacteria. Photoproduction of ammonia was monitored in the presence of L-methionine-D,L-sulfoximine, an inhibitor of glutamine synthetase activity. High yields of ammonia production, up to 400 .mu.mol and 100 .mu.mol mg chlorophyll-1 per 24 h, were obtained in batch reactors from polyvinyl foam immobilized A. azollae and M. laminosus, respectively, whereas control free-living cultures produced less than 10 .mu.mol ammonia under the same conditions. A similar yield increase was observed when the membrane permeability of alginate-immobilized A. azollae was increased by an acetone pretreatment. This suggests that the increase in ammonia production on immobilization in polyvinyl foam is at least partly related to changes in membrane permeability induced by the immobilization process itself as a consequence of cell-matrix interactions.