Modulation of inflammatory and profibrotic signaling in a rabbit model of acute phonotrauma using triamcinolone.
Modulation of inflammatory and profibrotic signaling in a rabbit model of acute phonotrauma using triamcinolone.
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DOI：
10.1177/0194599812440419
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Rousseau B
中科院分区：
文献类型：
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作者：
Hall JE;Suehiro A;Branski RC;Garrett CG;Rousseau B
探讨预防性曲安奈德调节急性声带炎性和促纤维化信号在急性声创伤的假设。在体兔发声模型。学术医疗中心。将40只新西兰白色种兔随机分配至4组之一：控制（无干预），无治疗（30分钟提高强度发声），假治疗（双侧喉内注射0 μg/25 μL曲安奈德，随后进行30分钟的提高强度发声），或类固醇治疗（双侧喉内注射400 μg/25 μL曲安奈德，随后进行30分钟的提高强度发声）。采用定量聚合酶链反应（qPCR）研究环氧化酶-2（考克斯-2）、白细胞介素（IL）-1β和转化生长因子（TGF）-β1的基因表达水平。结果显示考克斯-2的显著主效应（P = 0.002）。事后检验显示，未接受治疗的兔（15.10）比对照组（5.90; P <.001）具有更高的考克斯-2基因表达。治疗组之间的考克斯-2表达无显着差异。结果显示IL-1β的显著主效应（P < .001）。事后检验显示，未接受治疗的家兔（14.70）的IL-1β基因表达高于对照组（6.30）（P = .001）。治疗组间IL-1β基因表达无显著差异。治疗组和对照组之间TGF-β1基因表达无显著差异（P = 0.525）。鉴于相互矛盾的证据，进一步的研究是必要的，以调查声带类固醇注射之前和之后的诱发声创伤。在急性声损伤前立即预防性给予曲安西龙未导致考克斯-2、IL-1β和TGF-β1基因转录水平的显著变化。
To investigate the hypothesis that prophylactic triamcinolone modulates acute vocal fold inflammatory and profibrotic signaling during acute phonotrauma. In vivo rabbit phonation model. Academic medical center. Forty New Zealand white breeder rabbits were randomly assigned to 1 of 4 groups: control (no intervention), no treatment (30 minutes of raised intensity phonation), sham treatment (bilateral intralaryngeal triamcinolone acetonide injection at 0 μg/25 μL followed by 30 minutes of raised intensity phonation), or steroid treatment (bilateral intralaryngeal triamcinolone acetonide injection at 400 μg/25 μL followed by 30 minutes of raised intensity phonation). Quantitative polymerase chain reaction (qPCR) was used to investigate gene expression levels of cyclooxygenase-2 (COX-2), interleukin (IL)–1β, and transforming growth factor (TGF)–β1. Results revealed a significant main effect for COX-2 (P = .002). Post hoc testing revealed that rabbits receiving no treatment (15.10) had higher COX-2 gene expression than control (5.90; P <.001). There were no significant differences in COX-2 expression between treatment groups. Results revealed a significant main effect for IL-1β (P < .001). Post hoc testing revealed that rabbits receiving no treatment (14.70) had higher IL-1β gene expression than control (6.30) (P = .001). There were no significant differences in IL-1β gene expression between treatment groups. There were no significant differences in TGF-β1 gene expression (P = .525) between treatment and control groups. Given conflicting evidence, further studies are necessary to investigate vocal fold steroid injections prior to and following the induction of phonotrauma. Prophylactic administration of triamcinolone immediately prior to acute phonotrauma resulted in no significant changes in COX-2, IL-1β, and TGF-β1 gene transcript levels.