Involvement of glutathione in the enhanced renal excretion of methyl mercury in CFW Swiss mice.
Involvement of glutathione in the enhanced renal excretion of methyl mercury in CFW Swiss mice.
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DOI：
10.1016/0041-008x(85)90252-2
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发表时间：
1985-05
影响因子：
3.8
通讯作者：
K. M. Mulder;P. Kostyniak
中科院分区：
文献类型：
--
作者：
K. M. Mulder;P. Kostyniak
目前的研究试图确定机制升高尿甲基汞（MM）排泄率先前报道的CFW瑞士小鼠。对可能影响MM肾排泄的因素进行了菌株比较。MM向无机形式的生物转化似乎没有发挥重要作用。在体内或体外条件下，未观察到血浆和红细胞之间MM分布的显著菌株差异。低分子量组分中存在的总血浆MM百分比在CFW和CBA J菌株之间无统计学差异。肝脏、肾脏、全血和血浆中总还原非蛋白硫醇浓度的菌株比较显示无显著菌株差异。观察到血浆氧化型谷胱甘肽（GSSG）浓度的显著菌株差异。然而，还原型谷胱甘肽（GSH），谷胱甘肽（GS）的形式与MM相互作用的血浆浓度没有显着变化的菌株之间。CFW小鼠尿液中总谷胱甘肽（TGS）排泄率约为CBA J小鼠的2倍。CFW小鼠尿GS排泄量显著增加，同时该品系小鼠尿γ-谷氨酰转肽酶（γ-GTP）活性降低1.6倍。
The present studies attempted to identify the mechanism for the elevated urinar excretion rate for methyl mercury (MM) previously reported in CFW Swiss mice. Strain comparisons of factors which could conceivably influence renal excretion of MM were made. The biotransformation of MM to the inorganic form did not appear to play a significant role. No significant strain differences were observed in the distribution of MM between plasma and red cells under in vivo or in vitro conditions. The percentage of total plasma MM present in the low-molecular-weight fraction did not differ statistically between the CFW and CBA J strains. Strain comparisons of total reduced nonprotein thiol concentrations in liver, kidneys, whole blood, and plasma revealed no significant strain differences. A significant strain difference in plasma oxidized glutathione (GSSG) concentrations was observed. However, plasma concentrations of reduced glutathione (GSH), the form of glutathione (GS) which interacts with MM. did not significantly vary between the strains. The rate of total glutathione (TGS) excretion in urine was approximately 2-fold higher in CFW mice than in CBA J mice. The significantly higher urinary GS excretion in CFW mice was accompanied by a 1.6-fold lower urinary γ-glutamyltranspeptidase (γ-GTP) activity in this strain.