nanos gene control DNA mediates developmentally regulated transposition in the yellow fever mosquito Aedes aegypti
nanos gene control DNA mediates developmentally regulated transposition in the yellow fever mosquito Aedes aegypti
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DOI：
10.1073/pnas.0701515104
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发表时间：
2007-06-12
影响因子：
11.1
通讯作者：
James, Anthony A.
中科院分区：
文献类型：
--
作者：
Adelman, Zach N.;Jasinskiene, Nijole;James, Anthony A.
转座元件(TES)被认为是开发驱动系统通过媒介蚊子种群传播病原菌抗性基因的基础。利用昆虫胚系中特异表达的基因的转录和翻译控制DNA元件来介导转座，为减轻人们对目标载体物种中转基因行为的担忧，消除对非目标生物的影响提供了可能性。在这里，我们描述了成功地利用黄热病蚊子埃及伊蚊Nanos(Nos)正源基因的启动子和非翻译区来控制外源衍生的Mariner MOSL转座酶编码DNA的性别和组织特异性表达。转基因蚊子在接近或等于内源性一氧化氮合酶转录本的水平上大量表达转座酶基因，且仅在雌性生殖细胞中表达。此外，MOSL mRNA在发育中的卵母细胞中沉积，并在胚胎发育早期定位并维持在后极。重要的是，在研究的五个转基因品系中，有四个能够将第二个MOSL转基因动员到蚊子基因组中，这表明正在产生功能性转座酶。因此，nos控制序列有望成为基于TE的基因驱动系统的一部分。
Transposable elements (TEs) are proposed as a basis for developing drive systems to spread pathogen resistance genes through vector mosquito populations. The use of transcriptional and translational control DNA elements from genes expressed specifically in the insect germ line to mediate transposition offers possibilities for mitigating some of the concerns about transgene behavior in the target vector species and eliminating effects on nontarget organisms. Here, we describe the successful use of the promoter and untranslated regions from the nanos (nos) orthologous gene of the yellow fever mosquito, Aedes aegypti, to control sex- and tissue-specific expression of exogenously derived mariner Mosl transposase-encoding DNA. Transgenic mosquitoes expressed transposase mRNA in abundance near or equal to the endogenous nos transcript and exclusively in the female germ cells. In addition, Mosl mRNA was deposited in developing oocytes and localized and maintained at the posterior pole during early embryonic development. Importantly, four of five transgenic lines examined were capable of mobilizing a second Mosl transgene into the mosquito genome, indicating that functional transposase was being produced. Thus, the nos control sequences show promise as part of a TE-based gene drive system.