DNA methylation and histone acetylation: genotypic variations in hybrid poplars, impact of water deficit and relationships with productivity
DNA methylation and histone acetylation: genotypic variations in hybrid poplars, impact of water deficit and relationships with productivity
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DOI：
10.1051/forest/2009101
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发表时间：
2010-03-01
影响因子：
3
通讯作者：
Maury, Stephane
中科院分区：
文献类型：
--
作者：
Gourcilleau, Delphine;Bogeat-Triboulot, Marie-Beatrice;Maury, Stephane
一些关于一年生植物的报告已经表明，表观遗传修饰如DNA甲基化参与了它们对非生物胁迫的适应。然而，对于多年生植物，表观遗传修饰因子的基因型变异、它们与形态性状的可能相关性以及水分亏缺的影响尚未得到描述。对6个基因型的三角胡杨进行或不进行适度水分亏缺处理。通过测量植株高度、生物量和总叶面积等形态性状来表征两种条件下的生产力。在茎尖形态发生位点测定了DNA甲基化水平、组蛋白乙酰化水平以及相应酶的活性和同型异构体积累。观察了形态性状和表观遗传变量的基因型变异，并建立了它们之间的相关关系。在杂交杨树中检测到DNA甲基化基因型变异。在水分充足的条件下，DNA甲基化率与产量呈正相关。虽然所有基因型在适度缺水的情况下生长普遍下降，但发现DNA甲基化的基因型依赖性变异表明不同杂交品种的策略不同。
Several reports on annual plants have already shown the involvement of epigenetic modifiers such as DNA methylation in their adaptation to abiotic stresses.Nevertheless, the genotypic variations of epigenetic modifiers, their possible correlations with morphological traits and the impact of water deficit have not been described for perennial plants.Six genotypes of Populus deltoides x P. nigra were subjected or not to a moderate water deficit treatment. Various morphological traits such as the height of the plants, their biomass and the total leaf area were measured to characterize the productivity in both conditions. Levels of DNA methylation, histone acetylation and the activities and isoform accumulation of the corresponding enzymes were measured at the shoot apex, the site of morphogenesis. Genotypic variation was observed for the morphological traits and the epigenetic variables and correlations were established among them. Genotypic variation for DNA methylation was detected in hybrid poplars. A positive correlation was demonstrated between DNA methylation percentage and productivity under well watered conditions.While there was a general decrease of growth for all genotypes in response to a moderate water deficit, genotypic dependant variations of DNA methylation were found suggesting different strategies among hybrids.