Pet dogs potential transmitters of pathogenic Escherichia coli with resistance to antimicrobials
Pet dogs potential transmitters of pathogenic Escherichia coli with resistance to antimicrobials
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宠物狗是具有抗菌药物耐药性的致病性大肠杆菌的潜在传播者
DOI：
10.1007/s00203-020-01828-9
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发表时间：
2020
影响因子：
2.8
通讯作者：
C. Eslava
中科院分区：
文献类型：
--
作者：
X. Vega;A. Ubiarco;A. Verdugo;U. Hernández;A. Navarro;R. E. Ahumada;D. Ramírez;N. Hernández;C. Eslava
大肠杆菌是人类和动物正常生物群的一部分，然而，一些临床报告暗示大肠杆菌。大肠杆菌是人类和伴侣动物腹泻的病原体。因此，本研究的目的是了解圣刘易斯波托西市的伴侣犬是否被有毒的潜在有害的E定殖。大肠杆菌分析了30只犬的直肠拭子，其中13只有腹泻，17只无腹泻。对该菌株进行了系统发育和毒力基因分析。大肠杆菌分离物。此外，采用Kirby-Bauer试验分析了来自14个家庭的32种不同抗菌药物的敏感性。85株经鉴定为E.大肠杆菌在健康犬和腹泻犬中检出率为97%。来自健康狗的大肠杆菌分离株携带多个毒力基因，而来自腹泻动物的大肠杆菌分离株仅携带lyeaeA。在健康犬中，系统发育分析显示，57%和43%的E.大肠菌群分别属于弱毒群（A和B1）和强毒群（B2和D）。同时，对家犬进行了毒力测定，结果表明，69%的分离株为B2和D3毒力群。此外，E.两组犬均检出对β-内酰胺类、氨基糖苷类、四环素类、喹诺酮类和叶酸抑制剂耐药的大肠杆菌。E的存在。大肠杆菌无毒基因，表明这些菌株与动物的状况有关。最后，由于狗具有隐藏和传播危险的能力，所以必须特别注意对狗的小心处理。大肠杆菌
Escherichia colistrains are part of the normal biota of humans and animals; however, several clinical reports have implicatedE. colias the etiological agent of diarrhea in humans and companion animals. Thus, the aim of the present study was to know if companion dogs in the city of San Luis Potosi are colonized with virulent potentially harmfulE. colistrains. Rectal swabs from 30 dogs, 13 with and 17 without diarrhea were analyzed. Phylogenetic and virulence genes analysis was performed to theE. coliisolates. Additionally, the Kirby-Bauer test was used to analyze the sensitivity to 32 different antimicrobials from 14 families. Eighty-five isolates were identified asE. coliand detected in 97% of healthy and diarrheic dog samples.E. coliisolates from healthy dogs carried several virulence genes, in contrast with those from diarrheic animals that presented onlyeaeA. In healthy dogs, phylogenetic analysis showed that 57% and 43% ofE. coliisolates belonged to commensal (A and B1) and virulent (B2 and D) groups respectively. Meanwhile, diarrheic dogs showed that 69% of the isolates were identified as virulent B2 and D phylogroups. Moreover,E. coliresistant to β-lactams, aminoglycosides, tetracycline, quinolones, and folate inhibitors were detected in both groups of dogs. The presence ofE. coliwitheaeAvirulence gene in diarrheic dogs, suggest that these strains are associated with the animal´s condition. Finally, major attention must be drawn to the careful handling of dogs because of their capability to harbor and disseminate virulentE. colistrains.
影响因子：
3.6
作者：
Elliott, SJ;Wainwright, LA;Kaper, JB
通讯作者：
Kaper, JB