Chemoattractant and neutrophil degranulation activities related to interleukin-8 in vitreous fluid in uveitis and vitreoretinal disorders.
Chemoattractant and neutrophil degranulation activities related to interleukin-8 in vitreous fluid in uveitis and vitreoretinal disorders.
复制标题
与葡萄膜炎和玻璃体视网膜疾病玻璃体液中白细胞介素 8 相关的化学引诱剂和中性粒细胞脱粒活性。
DOI：
--
复制
发表时间：
1993
影响因子：
4.4
通讯作者：
A. Kijlstra
中科院分区：
文献类型：
--
作者：
J. D. Boer;C. Hack;A. Verhoeven;G. Baarsma;P. Jong;A. JosJ;M. Rademakers;Willemien A. E. J. de Vries;A. Rothova;A. Kijlstra
目的
研究细胞因子白细胞介素-8（IL-8）作为中性粒细胞的一种强趋化因子和激活剂，是否与葡萄膜炎患者眼内中性粒细胞浸润和脱颗粒有关。
方法
IL-8和弹性蛋白酶的测定与特定的酶联免疫测定法从69例患者的各种葡萄膜炎实体获得的玻璃体液样本。以非葡萄膜炎患者和眼库眼的玻璃体液为对照。用Boyden小室法检测玻璃体液的趋化活性。
结果
IL-8在葡萄膜炎患者的45%的玻璃体液样品和非葡萄膜炎患者的26%的玻璃体液样品中被检测到。IL-8水平超过100 pg/ml的玻璃体液样本对中性粒细胞具有趋化性。这种趋化活性可以用单克隆抗IL-8抗体阻断41%至79%。可检测到IL-8的葡萄膜炎患者玻璃体液中的弹性蛋白酶水平显著高于不可检测到IL-8的玻璃体液样品中的弹性蛋白酶水平。
结论
这些结果表明，IL-8通过吸引和脱粒中性粒细胞参与眼睛的炎症过程。这表明，这些过程有助于葡萄膜炎的组织破坏的发病机制。
PURPOSE
To investigate whether the cytokine interleukin-8 (IL-8), a strong chemoattractant and activator for neutrophils, is responsible for neutrophil infiltration and degranulation in the eye in uveitis.
METHODS
IL-8 and elastase were measured with specific enzyme-linked immunoassays in vitreous fluid samples obtained from 69 patients with various uveitis entities. Vitreous fluid of nonuveitis patients and eye bank eyes served as controls. The chemotactic activity of vitreous fluid was tested with the Boyden chamber technique.
RESULTS
IL-8 was detected in 45% of the vitreous fluid samples from uveitis patients and in 26% of vitreous fluid samples from nonuveitis patients. Vitreous fluid samples with IL-8 levels exceeding 100 pg/ml were chemotactic for neutrophils. This chemotactic activity could be blocked by 41% to 79% with a monoclonal anti-IL-8 antibody. Elastase levels in vitreous fluid of uveitis patients with detectable IL-8 were significantly higher than those in vitreous fluid samples with no detectable IL-8.
CONCLUSION
These results indicate that IL-8 participates in the inflammatory processes in the eye by attracting and degranulating neutrophils. It is suggested that these processes contribute to the pathogenesis of tissue destruction in uveitis.
登录
查看更多内容
视网膜色素上皮细胞响应白细胞介素 1 分泌白细胞介素 6。
影响因子：
4.4
作者：
Planck,SR;Dang,TT;Graves,D;Tara,D;Ansel,JC;Rosenbaum,JT
通讯作者：
Rosenbaum,JT
影响因子：
4.4
作者：
E. J. Campbell;E. K. Silverman;M. Campbell
通讯作者：
E. J. Campbell;E. K. Silverman;M. Campbell
葡萄膜炎患者房水中的白三烯。
DOI：
10.1001/archopht.1986.01050170112033
发表时间：
1986
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Parker,JA;Goetzl,EJ;Friedlaender,MH
通讯作者：
Friedlaender,MH
影响因子：
3.4
作者：
ELNER, VM;SCALES, W;STRIETER, RM
通讯作者：
STRIETER, RM