Characterization of 2‐Fluoro‐2’‐deoxyadenosine in Duplex, G‐Quadruplex and i‐Motif
Characterization of 2‐Fluoro‐2’‐deoxyadenosine in Duplex, G‐Quadruplex and i‐Motif
复制标题
双链体、G-四链体和 i-基序中 2-氟-2-脱氧腺苷的表征
DOI：
10.1002/cbic.202200222
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发表时间：
2022
期刊：
影响因子：
3.2
通讯作者：
Park Soyoung
中科院分区：
文献类型：
--
作者：
Hirashima Shingo;Sugiyama Hiroshi;Park Soyoung
在各种氟取代的生物分子中，2-氟腺嘌呤（2FA）及其衍生物作为治疗试剂、放射增敏剂和19 F NMR探针已被积极研究。尽管它们具有优异的性质，但含有2FA的DNA尚未得到很好的研究。为了对功能性核酸的开发的基本理解和未来应用，我们表征了典型右手DNA双链体、G-四链体和i-基序结构的含2FA的寡核苷酸。由于氟原子的小尺寸，2FA的性质类似于天然腺嘌呤，但它显示出由高电负性引起的独特特征。这项工作为2FA修饰的DNA的未来应用提供了有用的信息。
Among various kinds of fluorine‐substituted biomolecules, 2‐fluoroadenine (2FA) and its derivatives have been actively investigated as therapeutic reagents, radio‐sensitizers, and19F NMR probes. In spite of their excellent properties, DNA containing 2FA has not been studied well. For fundamental understanding and future applications to the development of functional nucleic acids, we characterized 2FA‐containing oligonucleotides for canonical right‐handed DNA duplex, G‐quadruplex, and i‐motif structures. Properties of 2FA were similar to native adenine due to the small size of the fluorine atom, but it showed unique features caused by high electronegativity. This work provides useful information for future application of 2FA‐modified DNA.