CHRONIC HEPATITIS-B VIRUS-INFECTION IN AN ANTI-HBC NONREACTIVE BLOOD-DONOR - VARIANT VIRUS OR DEFECTIVE IMMUNE-RESPONSE
CHRONIC HEPATITIS-B VIRUS-INFECTION IN AN ANTI-HBC NONREACTIVE BLOOD-DONOR - VARIANT VIRUS OR DEFECTIVE IMMUNE-RESPONSE
复制标题
DOI：
10.1002/hep.1840160106
复制
发表时间：
1992-07-01
期刊：
影响因子：
13.5
通讯作者：
ZELDIS, JB
中科院分区：
文献类型：
--
作者：
LEE, JH;PAGLIERONI, TG;ZELDIS, JB
研究了一名罕见的无症状慢性乙型肝炎病毒携带者（人白细胞抗原A24、Bw61、Bw62、Bw6、DRw11、DRw52、DQw7）的病毒序列和宿主免疫反应，该患者在5年的研究期间对HBc抗体一直无反应，ALT水平正常。从其血清中分离的病毒前核区和核心区DNA序列有7个沉默突变，导致adr HBsAg亚型氨基酸序列无变化。他的外周血T细胞和B细胞数量没有异常，也没有hbv特异性抑制T细胞。他的淋巴细胞在体外对植物血凝素、美洲商陆丝裂原、金黄色葡萄球菌和破伤风类毒素有增殖反应，但对重组HBcAg没有反应。与其他HBsAg携带者和乙肝病毒免疫个体不同，他的单核细胞没有吞下涂有HBcAg的珠粒。不能产生HBc抗体不是由于乙型肝炎病毒变异，而是由于这个无症状的HBsAg携带者的选择性免疫系统缺陷。
Viral sequence and host immune response were investigated in an unusual, asymptomatic chronic hepatitis B virus carrier (human leukocyte antigen type A24, Bw61, Bw62, Bw6, DRw11, DRw52, DQw7) who was consistently nonreactive for antibody to HBc and had a normal ALT level over a 5-yr study period. The precore and core region DNA sequences of virus isolated from his serum had seven silent mutations that resulted in no changes in the amino acid sequence of the adr HBsAg subtype. He had no abnormalities in the number of peripheral blood T or B cells and no HBcAg-specific suppressor T cells. His lymphocytes proliferated in vitro in response to phytohemagglutinin, pokeweed mitogen, Staphylococcus aureus and tetanus toxoid but not to recombinant HBcAg. Unlike other HBsAg carriers and hepatitis B virus-immune individuals, his monocytes did not ingest beads coated with HBcAg. Failure to produce antibody to HBc was not due to an hepatitis B virus variant but to a selective immune system defect in this asymptomatic HBsAg carrier.