Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields.
Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields.
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优化高磁场下异质混合物中稀释蛋白质的 ssNMR 实验。
DOI：
10.1002/mrc.2146
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Long,JoannaR
中科院分区：
文献类型：
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作者：
McNeill,SethA;Gor'kov,PeterL;Struppe,Jochem;Brey,WilliamW;Long,JoannaR
高磁场下的固态NMR光谱被证明是结构生物学中的一种有效技术，特别是对于不适合传统X射线和溶液NMR方法的蛋白质。可以选择几个参数来提供最佳灵敏度，提高样品稳定性，并确保蛋白质样品ssNMR测量的生物相关性。这些包括样品条件的选择，NMR探针设计，和脉冲实验的设计。在这里，我们展示和评估几个工程和实验方法追求测量稀释蛋白质的异质混合物。版权所有© 2007约翰威利父子有限公司。
Solid‐state NMR spectroscopy at high magnetic fields is proving to be an effective technique in structural biology, particularly for proteins which are not amenable to traditional X‐ray and solution NMR approaches. Several parameters can be selected to provide optimal sensitivity, improve sample stability, and ensure biological relevance for ssNMR measurements on protein samples. These include selection of sample conditions, NMR probe design, and design of pulse experiments. Here, we demonstrate and evaluate several engineering and experimental approaches for pursuing measurements on dilute proteins in heterogeneous mixtures. Copyright © 2007 John Wiley & Sons, Ltd.