Behavior-related pauses in simple-spike activity of mouse Purkinje cells are linked to spike rate modulation.
Behavior-related pauses in simple-spike activity of mouse Purkinje cells are linked to spike rate modulation.
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DOI：
10.1523/jneurosci.4969-11.2012
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发表时间：
2012-06-20
期刊：
影响因子：
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通讯作者：
Heck DH
中科院分区：
文献类型：
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作者：
Cao Y;Maran SK;Dhamala M;Jaeger D;Heck DH
哺乳动物小脑中的浦肯野细胞 (PC) 表现出高频自发活动，平均峰值速率在 30 至 200 Hz 之间。小脑核 (CN) 神经元接收来自许多 PC 的汇聚输入，从而产生连续的抑制输入。据推测，PC 活动的暂停会触发 CN 尖峰活动的增加。从这一假设得出的预测是，PC 简单尖峰活动的暂停代表相关的行为或感觉事件。在这里，我们询问 PC 与液体舔舐或呼吸相关的简单尖峰活动中的暂停是否在代表行为信息方面发挥特殊作用。这两种行为在小脑 PC 简单尖峰活动中得到了广泛的体现。我们记录了头部固定小鼠蚓部和单叶中的 PC 活动，同时监测舔舐和呼吸行为。使用互相关和格兰杰因果分析，我们检查了短 ISI 与行为的时间关系是否与长 ISI 或暂停不同。在舔舐和呼吸相关的 PC 中，在低速率简单尖峰活动期间，会发生与行为相关的简单尖峰暂停。格兰杰因果关系分析揭示了简单的尖峰暂停和行为之间的因果关系。然而，通过对具有伽玛 ISI 分布的替代尖峰序列的分析获得了相同的结果，该分布被构建为匹配行为相关浦肯野细胞的速率调节。因此，我们的结果表明，简单尖峰活动中发生的暂停并不代表超出简单尖峰速率调节范围的有关行为或感觉事件的附加信息。
Purkinje cells (PCs) in the mammalian cerebellum express high frequency spontaneous activity with average spike rates between 30 and 200 Hz. Cerebellar nuclear (CN) neurons receive converging input from many PCs resulting in a continuous barrage of inhibitory inputs. It has been hypothesized that pauses in PC activity trigger increases in CN spiking activity. A prediction derived from this hypothesis is that pauses in PC simple spike activity represent relevant behavioral or sensory events. Here we asked whether pauses in the simple spike activity of PCs related to either fluid licking or respiration, play a special role in representing information about behavior. Both behaviors are widely represented in cerebellar PC simple spike activity. We recorded PC activity in the vermis and lobus simplex of head fixed mice while monitoring licking and respiratory behavior. Using cross correlation and Granger causality analysis we examined whether short ISIs had a different temporal relation to behavior than long ISIs or pauses. Behavior related simple spike pauses occurred during low-rate simple spike activity in both licking and breathing related PCs. Granger causality analysis revealed causal relationships between simple spike pauses and behavior. However, the same results were obtained from an analysis of surrogate spike trains with gamma ISI distributions constructed to match rate modulations of behavior related Purkinje cells. Our results therefore suggest that the occurrence of pauses in simple spike activity does not represent additional information about behavioral or sensory events that goes beyond the simple spike rate modulations.