Enhanced renal IGF-I expression following partial kidney infarction.
Enhanced renal IGF-I expression following partial kidney infarction.
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DOI：
10.1152/ajprenal.1993.264.6.f963
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发表时间：
1993-06
期刊：
影响因子：
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通讯作者：
S. Rogers;S. Miller;M. R. Hammerman
中科院分区：
文献类型：
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作者：
S. Rogers;S. Miller;M. R. Hammerman
肾脏胰岛素样生长因子(IGF)-I在肾脏肿块切除后残留的组织中表达增强。为了研究单侧部分梗死区肾质量减轻后肾脏IGF-I的表达情况，我们测定了未行(1/2NX)或(1 1/2NX)对侧肾切除的单侧梗死区的非梗死区(残留)肾组织中IGF-I的表达水平。1/2NX后残余肾组织中IGF-I水平显著升高，在肾质量减少后第3天达到峰值，然后恢复到基线水平。在第3天，IGF-I mRNA的稳态水平也升高，表明IGF-I的增加是合成增强的结果。在1 1/2NX后，IGF-I的提取和IGF-I的mRNA表达也出现了类似的模式。1 1/2NX后3d残肾组织中IGF-I水平与1/2NX后3d残肾组织中IGF-I水平无明显差异，但均高于单侧肾切除后3d对侧肾组织IGF-I水平。因此，IGF-I水平与肾质量本身减少的程度无关。我们得出结论，部分肾梗死可刺激IGF-I的表达。在解释慢性肾功能衰竭残肾模型的研究结果时，必须考虑肾脏IGF-I的升高。
Renal insulin-like growth factor (IGF)-I expression is enhanced in tissue that remains following removal of kidney mass. To characterize the expression of renal IGF-I after reduction of kidney mass by partial unilateral infarction, we measured levels of IGF-I extracted from noninfarcted (remnant) renal tissue that remained after one-half unilateral kidney infarction that was performed without (1/2NX) or with (1 1/2NX) contralateral nephrectomy. Levels of IGF-I extracted from remnant renal tissue after 1/2NX increased significantly, peaking on day 3 after renal mass reduction, and then returned toward baseline. Steady-state levels of IGF-I mRNA were also elevated on day 3, suggesting that the increase in IGF-I results from enhanced synthesis. A similar pattern of increased extracted IGF-I and elevated IGF-I mRNA occurred after 1 1/2NX. Levels of IGF-I extracted from remnant renal tissue 3 days after 1 1/2NX were not higher than levels extracted from remnant tissue 3 days after 1/2NX, and both were higher than levels of IGF-I extracted from contralateral kidneys 3 days after unilateral nephrectomy. Therefore, levels of IGF-I did not correlate with the extent of reduction of renal mass per se. We conclude that partial renal infarction provides a stimulus to enhance IGF-I expression. Increased renal IGF-I must be considered in the interpretation of findings originating from use of remnant kidney models of chronic renal failure.