Molecular Identification and Analysis of Cd-Responsive MicroRNAs in Rice
Molecular Identification and Analysis of Cd-Responsive MicroRNAs in Rice
复制标题
水稻镉响应性microRNA的分子鉴定与分析
DOI：
10.1021/jf401359q
复制
发表时间：
2013-11-27
影响因子：
6.1
通讯作者：
Zhu, Cheng
中科院分区：
文献类型：
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作者：
Ding, Yanfei;Qu, Aili;Zhu, Cheng
镉（Cd）是一种非必需重金属，对植物具有很高的毒性。MicroRNAs （miRNAs）是一类在植物非生物胁迫反应中起重要作用的小分子非编码rna。为了研究miRNA是否在Cd胁迫响应中起作用，利用微阵列技术监测了水稻在Cd胁迫下的miRNA表达谱。共鉴定出12个先前预测的cd响应性新mirna，其中4个在实验中得到进一步验证。总共有44个靶基因被预测为cd响应mirna，其中许多似乎调节介导环境应激的基因网络。几个靶基因被证实显示mirna的相互调控。还采用转基因方法确定了mirna在水稻对Cd胁迫反应中的作用。过表达miR192抑制Cd胁迫下种子萌发和幼苗生长。这些结果暗示了新mirna在水稻耐Cd性调控中的作用。
Cadmium (Cd) is a non-essential heavy metal with high toxicity to plants. MicroRNAs (miRNAs) are a class of small non-coding RNAs that play important roles in plant abiotic stress responses. To investigate whether miRNAs function in Cd stress response, miRNA expression profiles in rice (Oryza sativa) under Cd stress were monitored using microarray assays. A total of 12 Cd-responsive novel miRNAs predicted previously were identified, of which 4 were further validated experimentally. A total of 44 target genes were predicted for the Cd-responsive miRNAs, many of which appeared to regulate gene networks mediating environmental stresses. Several target genes were validated to show a reciprocal regulation by miRNAs. A transgenic approach was also used to determine the role of miRNAs in rice response to Cd stress. Overexpression of miR192 retarded seed germination and seedling growth under Cd stress. These results implied the role of novel miRNAs in the involvement of Cd tolerance of rice.