PALMITATE-DERIVATIZED ANTIBODIES CAN FUNCTION AS SURROGATE RECEPTORS FOR MEDIATING SPECIFIC CELL-CELL INTERACTIONS
PALMITATE-DERIVATIZED ANTIBODIES CAN FUNCTION AS SURROGATE RECEPTORS FOR MEDIATING SPECIFIC CELL-CELL INTERACTIONS
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DOI：
10.1016/0022-1759(89)90351-7
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发表时间：
1989-11-30
影响因子：
2.2
通讯作者：
PEACOCK, JS
中科院分区：
文献类型：
--
作者：
COLSKY, AS;PEACOCK, JS
观察到外源提供的药物可以绕过内在的识别机制，促进细胞的结合，这导致了对细胞功能机制的有价值的见解。目前可用的细胞偶联剂的一个共同特征是它们依赖于两种细胞类型的内源性膜分子作为细胞相互作用的锚。在这份报告中，我们描述了一种将棕榈酸酯衍生的抗体分子整合到细胞膜上的方法，在细胞膜上，它们起到“替代受体”(SR)的作用，介导特定的细胞相互作用。在这个体系中，SR通过将棕榈酸酯碳链插入磷脂双层的外层小叶而连接到质膜上。因此，棕榈酸酯锚在抗体依赖的细胞结合中绕过了对FCR或其他内源性膜蛋白的要求。由于这种类似于磷脂酰肌醇(PI)锚定蛋白的附着模式，SR介导的细胞相互作用很可能使人想起天然受体诱导的结合，使SR能够在细胞-细胞界面的受体-配体相互作用中与内源性靶标识别结构合作。
The observation that exogenously supplied agents can bypass intrinsic recognition mechanisms and facilitate cellular conjugation has led to valuable insights into the mechanisms of cell function. A common feature of currently available cellular conjugation agents is their reliance on endogenous membrane molecules on both cell types as anchors for cellular interactions. In this report, we describe a method for incorporating palmitate-derivatized antibody molecules onto cell membranes where they function as ''surrogate receptors'' (SR) for mediating specific cellular interactions. In this system, SR are attached to the plasma membrane by insertion of the palmitate hydrocarbon chains into the outer leaflet of the phospholipid bilayer. Therefore, the palmitate anchor bypasses the requirement for FcR or other endogenous membrane proteins in antibody-dependent cellular conjugation. Due to this mode of attachment, which is similar to that of phosphatidylinositol (PI) anchored proteins, SR-mediated cellular interactions are likely to be reminiscent of native receptor-induced conjugation, enabling SR to cooperate with endogenous target recognition structures in receptor-ligand interactions at the cell-cell interface.