Metal-induced energy transfer for live cell nanoscopy
Metal-induced energy transfer for live cell nanoscopy
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DOI：
10.1038/nphoton.2013.345
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发表时间：
2014-02-01
期刊：
影响因子：
35
通讯作者：
Enderlein, Joerg
中科院分区：
文献类型：
--
作者：
Chizhik, Alexey I.;Rother, Jan;Enderlein, Joerg
福斯特共振能量转移（FRET）1的发现彻底改变了我们使用荧光成像在纳米尺度上测量分子间和分子内距离的能力。这种现象是基于电磁场介导的能量转移，从一个光激发供体到一个受体。我们用金属膜代替受体分子，并使用测量的从供体分子到金属表面等离子体的能量转移效率(2)来准确地推断分子与金属之间的距离。与FRET一样，这使得以纳米精度定位发射器成为可能，但有效能量转移发生的距离范围比传统FRET大一个数量级。这创造了一种在分子尺度上定位荧光实体的新方法，距离范围超过100纳米。我们通过分析活细胞的基底脂质膜来证明这种方法的力量。
The discovery of Forster resonance energy transfer (FRET)1 has revolutionized our ability to measure inter- and intramolecular distances on the nanometre scale using fluorescence imaging. The phenomenon is based on electromagnetic-field-mediated energy transfer from an optically excited donor to an acceptor. We replace the acceptor molecule with a metallic film and use the measured energy transfer efficiency from donor molecules to metal surface plasmons(2) to accurately deduce the distance between the molecules and metal. Like FRET, this makes it possible to localize emitters with nanometre accuracy, but the distance range over which efficient energy transfer takes place is an order of magnitude larger than for conventional FRET. This creates a new way to localize fluorescent entities on a molecular scale, over a distance range of more than 100 nm. We demonstrate the power of this method by profiling the basal lipid membrane of living cells.