Detection of multidrug-resistant Salmonella enterica serovar typhimurium phage types DT102, DT104, and U302 by multiplex PCR
Detection of multidrug-resistant Salmonella enterica serovar typhimurium phage types DT102, DT104, and U302 by multiplex PCR
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DOI：
10.1128/jcm.00171-06
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发表时间：
2006-07-01
影响因子：
9.4
通讯作者：
Chu, Chishih
中科院分区：
文献类型：
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作者：
Chiu, Cheng-Hsun;Su, Lin-Hui;Chu, Chishih
鼠伤寒沙门氏菌是人类和动物非伤寒沙门氏菌病的常见病因。多药耐药鼠伤寒血清型噬菌体DT 104型出现于1990年代，已在许多国家广泛分布。从1997年至2003年，从台湾三家主要医院收集了104株沙门氏菌血清群B的临床分离株，并通过多重PCR检测针对耐药基因和毒力质粒的spv基因。共有51个分离株（49%）对所有药物（ACSSuT [对氨苄青霉素、氯霉素、链霉素、磺胺和四环素的耐药性]）均具有耐药性，并且所有分离株均含有整合子的1.25 kb PCR片段，该片段是43 kb沙门氏菌基因组岛1（SGI 1）的一部分。第二组对SSu耐药（28%），第三组对所有五种药物敏感（13%）。用H抗血清通过试管凝集法对59株分离株进行血清型鉴定。在59株鼠伤寒血清型分离株中，54株（91.5%）检出毒力质粒。大多数（94.1%）具有ACSSuT耐药模式的沙门氏菌血清群B分离株携带毒力质粒。噬菌体分型鉴定了三种主要的噬菌体类型：DT 104、DT 120和U302。通过脉冲场凝胶电泳分析的菌株显示6种基因型。我们在DT 104菌株中发现了两种基因型，在DT 120中发现了两种基因型，在U302中发现了另外两种基因型。双相血清型（4，5，12：i：-）的存在增加了确定多重耐药沙门氏菌血清群B分离株血清型的难度。然而，多重PCR设计在本研究中似乎是有效的，有用的ACSSuT型血清型鼠伤寒与SGI 1的快速鉴定，无论其噬菌体类型。
Salmonella enterica serovar Typhimurium is a common cause of nontyphoidal salmonellosis in humans and animals. Multidrug-resistant serovar Typhimurium phage type DT104, which emerged in the 1990s, has become widely distributed in many countries. A total of 104 clinical isolates of Salmonella serogroup B were collected from three major hospitals in Taiwan during 1997 to 2003 and were examined by a multiplex PCR targeting the resistance genes and the spv gene of the virulence plasmid. A total of 51 isolates (49%) were resistant to all drugs (ACSSuT [resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline]), and all contained a 1.25-kb PCR fragment of integron that is part of the 43-kb Salmonella genomic island 1 (SGI1). The second group was resistant to SSu (28%), and the third was susceptible to all five drugs (13%). Fifty-nine isolates were serotyped to be serovar Typhimurium by the tube agglutination method using H antisera. The virulence plasmid was found in 54 (91.5%) of the 59 serovar Typhimurium isolates. A majority (94.1%) of the Salmonella serogroup B isolates with the ACSSuT resistance pattern harbored a virulence plasmid. Phage typing identified three major phage types: DT104, DT120, and U302. Analysis of the isolates by pulsed-field gel electrophoresis showed six genotypes. We found two genotypes in DT104 strains, two in DT120, and the other two in U302. The presence of a monophasic serovar (4,5,12:i:-) has added difficulty in the determination of the serovars of multidrug-resistant Salmonella serogroup B isolates. Nevertheless, the multiplex PCR devised in the present study appears to be efficient and useful in the rapid identification of ACSSuT-type serovar Typhimurium with SGI1, irrespective of their phage types.