Altered mobility of polydeoxyribonucleotides in high resolution polyacrylamide gels due to removal of terminal phosphates.
Altered mobility of polydeoxyribonucleotides in high resolution polyacrylamide gels due to removal of terminal phosphates.
复制标题
由于末端磷酸盐的去除，聚脱氧核糖核苷酸在高分辨率聚丙烯酰胺凝胶中的迁移率发生改变。
DOI：
10.1093/nar/9.24.6787
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发表时间：
1981
影响因子：
14.9
通讯作者：
Clayton,DA
中科院分区：
文献类型：
--
作者：
Tapper,DP;Clayton,DA
我们比较了一系列多核苷酸的电泳迁移率，这些多核苷酸的差异仅在于是否存在末端磷酸盐。正如预期的那样，去除单个末端磷酰基残基对电泳迁移率的影响取决于多核苷酸的大小，因此不是恒定的。从短于30个核苷酸的多核苷酸去除磷酰基残基会降低一个核苷酸的迁移率。在30至50个核苷酸之间，迁移率的降低约为半个核苷酸，而在50个核苷酸以上的大小，磷酸盐去除的效果接近零。
We have compared the electrophoretic mobility of a series of polynucleotides differing solely by the presence or absence of a terminal phosphate. As expected, the effect of removal of a single terminal phosphoryl residue on electrophoretic mobility is dependent on the size of the polynucleotide and therefore is not constant. Removal of a phosphoryl residue from polynucleotides shorter than 30 nucleotides reduces the mobility the equivalent of one nucleotide. Between 30 and 50 nucleotides the reduction in mobility is approximately one-half a nucleotide, while above 50 nucleotides in size the effect of phosphate removal approaches zero.