GASTRIN-RELEASING PEPTIDE IN HUMAN NASAL-MUCOSA
GASTRIN-RELEASING PEPTIDE IN HUMAN NASAL-MUCOSA
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DOI：
10.1172/jci114577
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发表时间：
1990-04-01
影响因子：
15.9
通讯作者：
KALINER, M
中科院分区：
文献类型：
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作者：
BARANIUK, JN;LUNDGREN, JD;KALINER, M
胃泌素释放肽(GRP)是蛙皮素的一种哺乳动物形式，由27个氨基酸组成，在人下鼻甲粘膜中进行了研究。放射免疫法测定粘膜GRP含量为0.60。+-。0.25pmol/g组织(n=9例；平均+-。扫描电子显微镜)。间接免疫组织化学免疫金法检测到的GRP免疫反应神经主要分布于小肌动脉、小动脉、静脉窦和粘膜下腺泡间。放射自显影测定的~(125)I-GRP结合部位定位于鼻黏膜上皮和粘膜下腺。没有对船只的约束。在短期外植体培养中研究GRP对粘膜下腺产物释放的影响。GRP(10微米)显著刺激浆液细胞特异性产物乳铁蛋白和[~3H]氨基葡萄糖标记的糖偶联物的释放，后者是上皮杯状细胞和粘膜下腺细胞的产物。这些结果表明，神经纤维释放的GRP可能作用于腺体GRP受体，引起粘膜下腺和上皮的糖共轭释放和浆膜细胞的乳铁蛋白释放，但GRP可能不影响血管通透性。
Gastrin-releasing peptide (GRP), the 27 amino acid mammalian form of bombesin, was studied in human inferior turbinate nasal mucosa. The GRP content of the mucosa measured by radioimmunoassay was 0.60 .+-. 0.25 pmol/g tissue (n = 9 patients; mean .+-. SEM). GRP-immunoreactive nerves detected by the immunogold method of indirect immunohistochemistry were found predominantly in small muscular arteries, arterioles, venous sinusoids, and between submucosal gland acini. 125I-GRP binding sites determined by autoradiography were exclusively and specifically localized to nasal epithelium and submucosal glands. There was no binding to vessels. The effects of GRP on submucosal gland product release were studied in short-term explant culture. GRP (10 .mu.M) significantly stimulated the release of the serous cell-specific product lactoferrin, and [3H]glucosamine-labeled glycoconjugates which are products of epithelial goblet cells and submucosal gland cells. These observations indicate that GRP released from nerve fibers probably acts on glandular GRP receptors to induce glycoconjugate release from submucosal glands and epithelium and lactoferrin release from serous cells, but that GRP would probably not affect vascular permeability.