Transplantation of endothelial progenitor cells into the lung to alleviate pulmonary hypertension in dogs
Transplantation of endothelial progenitor cells into the lung to alleviate pulmonary hypertension in dogs
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DOI：
10.1089/1076327041348563
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发表时间：
2004-05-01
影响因子：
--
通讯作者：
Shimizu, Y
中科院分区：
文献类型：
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作者：
Takahashi, M;Nakamura, T;Shimizu, Y
原发性肺动脉高压（PPH）仍然是一种难治性疾病，尽管进行任何治疗，患者病情都会恶化。我们假设肺部的新生血管形成可以增加肺循环中血管床的体积，从而减少肺动脉高压（PH）的发生。内皮祖细胞（EPC）可能是新生血管形成的潜在细胞来源。我们检查了 EPC 移植到患有脱氢野百合碱诱导的 PH 的狗肺部的影响。使用支气管镜向 PH 模型狗的肺实质注射来自外周血（实验，n=4）或培养基（对照，n=3）的离体扩增的自体 EPC。 EPC移植显着改善了平均肺动脉压、心输出量和肺血管阻力。组织学评估显示小肺动脉内侧厚度的改善和肺组织的新血管形成。这些结果表明，将 EPC 移植到肺部可有效预防犬脱氢野百合碱诱导的 PH 的进展，并为 PPH 提供一种新的治疗选择。
Primary pulmonary hypertension (PPH) is still a refractory disease, and patients deteriorate despite any treatment. We hypothesized that neovascularization in the lung could increase the volume of the vascular bed in the pulmonary circulation and thus reduce the development of pulmonary hypertension (PH). Endothelial progenitor cells (EPCs) might be a potential cell source for neovascularization. We examined the effects of EPC transplantation into the lungs of dogs with dehydromonocrotaline-induced PH. The lung parenchyma of PH model dogs was injected with ex vivo-expanded, autologous EPCs originated from peripheral blood (experiments, n=4) or culture medium (control, n=3), using a bronchoscope. EPC transplantation gave significant improvements in mean pulmonary artery pressure, cardiac output, and pulmonary vascular resistance. Histological evaluation revealed both improvement in the medial thickness of the small pulmonary artery and neovascularization of the lung tissue. These results indicate that EPC transplantation into the lung is effective at preventing the progression of dehydromonocrotaline-induced PH in dogs, and suggest a new therapeutic option for PPH.