A new experimental in vitro culture medium for cultivation of Leishmania species
A new experimental in vitro culture medium for cultivation of Leishmania species
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DOI：
10.1128/jcm.35.9.2430-2431.1997
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发表时间：
1997-09-01
影响因子：
9.4
通讯作者：
Ozbilgin, A
中科院分区：
文献类型：
--
作者：
Limoncu, ME;Balcioglu, IC;Ozbilgin, A
用一种新的液体培养基进行了利什曼原虫前鞭毛体的体外培养试验，以获得大量用于血清学研究的生物体。从Novy-MacNeal-Nicolle（NNN）培养基中取出的婴儿利什曼原虫和热带利什曼原虫前鞭毛体的数量在第8天结束时达到1 × 10（7）/ml。而在NNN培养基中，微生物数量仅达到5 × 10（6）/ml。在10次连续传代后，与其他市售液体培养基相比，所制备的培养基被评价为相当便宜、简单和成功。
A new liquid culture medium prepared with chemicals that can be obtained economically and commercially was tested in in vitro cultivation of Leishmania promastigotes to obtain a large number of organisms to use in serological studies, The number of Leishmania infantum and Leishmania tropica promastigotes taken from Novy-MacNeal-Nicolle (NNN) medium reached 1 x 10(7)/ml at the end of the 8th day In our nem medium, though in NNN medium the number of organisms reached only 5 x 10(6)/ml. After 10 subsequent passages, the culture medium prepared was evaluated as being quite inexpensive, simple, and successful compared with other commercially available liquid culture media.