PLASMID-CONTROLLED COLONIZATION FACTOR ASSOCIATED WITH VIRULENCE IN ESCHERICHIA-COLI ENTEROTOXIGENIC FOR HUMANS
PLASMID-CONTROLLED COLONIZATION FACTOR ASSOCIATED WITH VIRULENCE IN ESCHERICHIA-COLI ENTEROTOXIGENIC FOR HUMANS
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DOI：
10.1128/iai.12.3.656-667.1975
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
GORBACH, SL
中科院分区：
文献类型：
--
作者：
EVANS, DG;SILVER, RP;GORBACH, SL
从一例霍乱样腹泻患者中分离到一株产肠毒素大肠杆菌（E. coli菌株H-10407）具有表面相关的定殖因子。定植表现为小接种物（10（5）个细菌）在幼兔肠道中达到大（10（9）个）群体的能力，同时伴有肠道反应。实验室通过的E.大肠杆菌H-10407，命名为H-10407-P，在幼兔中没有表现出种群增加，也没有诱导腹泻。E.大肠杆菌H-10407和H-10407-P在幼兔和成年兔中产生相当的肠毒性反应。用热灭活和活细胞E. coliH-10407-P，该特异性吸附血清能保护幼兔免受活E. coliH-10407，但血清不具有杀菌活性。抗定殖因子血清不凝集E. coliK-12具有大肠杆菌特有的K88定殖因子。猪大肠杆菌。电镜观察表明，E. coliH-10407而非H10407-具有菌毛样的表面结构，能与特异吸附的抗定植因子抗血清凝集。E. coliH-10407具有三种质粒脱氧核糖核酸，分别为60 × 10 ~（6）、42 × 10 ~（6）和3.7 × 10 ~（6）道尔顿。E. coliH-10407-P只含有42 × 10（6）-和3.7 × 10（6）-道尔顿质粒。特异性H-10407表面相关抗原的自发丧失伴随着60 X 10（6）-道尔顿物质的质粒脱氧核糖核酸的丧失和定殖能力的丧失。因此，E.大肠杆菌定殖因子是一种毒力因子，它在天然存在的大肠杆菌中可能起重要的和可能必要的作用。大肠杆菌肠毒性腹泻。
An enterotoxin-producing strain of Escherichia coli isolated from a case of cholera-like diarrhea (E. coli strain H-10407) was found to possess a surface-associated colonization factor. Colonization was manifested as the ability of small inocula (10(5) bacteria) to attain large (10(9)) populations in the infant rabbit intestine with a concomitant diarrheal response. A laboratory-passed derivative of E. coli H-10407, designated H-10407-P, failed to exhibit an increase in population in the infant rabbit and also failed to induce diarrhea. Cell-free culture supernatant fluids of E. coli H-10407 and H-10407-P produced equivalent enterotoxic responses in infant and in adult rabbits. Specific anti-colonization factor antiserum was produced by adsorbing hyperimmune anti-H-10407 serum with both heat-killed and living cells E. coli H-10407-P. This specific adsorbed serum protected infant rabbits from challenge with living E. coli H-10407 although the serum did not possess bactericidal activity. The anti-colonization factor serum did not agglutinate a strain of E. coli K-12 possessing the K88 colonization factor peculiar to E. coli enterotoxigenic for swine. By electron microscopy it was demonstrated that E. coli H-10407, but not H10407-, possessed pilus-like surface structures which agglutinated with the specific adsorbed (anti-colonization factor) antiserum. E. coli H-10407 possessed three species of plasmid deoxyribonucleic acid, measuring 60 X 10(6), 42 X 10(6), and 3.7 X 10(6) daltons, respectively. E. coli H-10407-P possessed only the 42 X 10(6)- and the 3.7 X 10(6)-dalton plasmid species. Spontaneous loss of the specific H-10407 surface-associated antigen was accompanied by loss of the 60 X 10(6)-dalton species of plasmid deoxyribonucleic acid and loss of colonizing ability. Thus, it is concluded that the E. coli colonization factor described here is a virulence factor which may play an important and possibly essential role in naturally occurring E. coli enterotoxic diarrhea in man.