Genetic analysis of multi-drug resistance and the clonal dissemination of β-lactam resistance in Salmonella Infantis isolated from broilers
Genetic analysis of multi-drug resistance and the clonal dissemination of β-lactam resistance in Salmonella Infantis isolated from broilers
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DOI：
10.1016/j.vetmic.2009.07.007
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发表时间：
2010-01-06
影响因子：
3.3
通讯作者：
Okamoto, Karoku
中科院分区：
文献类型：
--
作者：
Shahada, Francis;Sugiyama, Haruhisa;Okamoto, Karoku
进行了一项流行病学研究，以调查发病率和抗菌药物耐药决定因素的特征，分析质粒谱，并建立日本南部肉鸡婴儿沙门氏菌β-内酰胺耐药分离株之间的遗传关系。在2004-2006年期间，从44个饲养场的56个鸡群中共回收了120个分离株。耐药率依次为：氨苄西林24%，头孢噻吩23%，头孢西丁0%，头孢他啶11%，头孢噻肟11%，氯霉素0%，卡那霉素7.5%，氧氟沙星20%，土霉素、链霉素、磺胺甲恶唑100%，甲氧苄啶75%。2004-2006年bla（TEM）编码的β-内酰胺类耐药发生率显著高于1998-2003年（P < 0.001）。BlnI消化的PFGE模式产生了两个相关的集群，涉及β-内酰胺耐药的传播。观察到两种类型的质粒图谱，并观察到两种ca. 50和180 kb大小的β-内酰胺耐药分离株携带。链霉素耐药性由aadA 1（n = 116）、aadA 1-aadA 2（n = 1）和aadA 1-strA-strB（n = 3）赋予。aphA 1（n = 9，100%）、tetA（n = 120，100%）、sul 1（n = 120，100%）和dfrA 5（n = 90，100%）分别对卡那霉素、土霉素、磺胺甲恶唑和甲氧苄啶产生耐药性。检测到两种类型的1类整合子：1.0 kb（n = 120）和1.0/1.5 kb（n = 3）。在aadA 1-strA-strB基因组合的菌株中发现了1.0/1.5kb的整合子。第一次，所有的S。Infantis菌株表现出耐药性至少三类抗菌药物和肠道的健康家禽是一个水库的广谱头孢菌素耐药菌株的血清型Infantis。(C)2009爱思唯尔有限公司版权所有。
An epidemiologic study was conducted to investigate the incidence and characterize the antimicrobial resistance determinants, analyzing plasmid profiles, and establishing the genetic relationship among beta-lactam-resistant isolates of Salmonella Infantis from broilers in Southern Japan. A total of 120 isolates were recovered from 56 flocks belonging to 44 holdings during 2004-2006. The percentages of resistance were as follows: ampicillin (24%), cephalothin (23%), cefoxitin (0%), ceftazidime (11%), cefotaxime (11%), chloramphenicol (0%), kanamycin (7.5%), ofloxacin (20%), oxytetracycline, streptomycin and sulfamethoxazole (100%) and trimethoprim (75%). The incidence of bla(TEM)-encoded beta-lactam resistance in 2004-2006 was significantly higher than in 1998-2003 (P < 0.001). BlnI-digested PFGE patterns generated two related clusters implicated in the dissemination of beta-lactam resistance. Two types of plasmid profiles were observed and two plasmids of ca. 50 and 180-kb size were carried by beta-lactam-resistant isolates. Streptomycin resistance was conferred by aadA1 (n = 116), aadA1-aadA2 (n = 1), and aadA1-strA-strB (n = 3). Resistances to kanamycin, oxytetracycline, sulfamethoxazole and trimethoprim were conferred by aphA1 (n = 9, 100%), tetA (n = 120, 100%) sul1 (n = 120, 100%) and dfrA5 (n = 90, 100%), respectively. Two types of class 1 integrons were detected: 1.0 kb (n = 120) and, 1.0/1.5 kb (n = 3). Integrons of 1.0/1.5 kb were found in isolates with the aadA1-strA-strB gene combination. For the first time, all S. Infantis isolates showed resistance to at least three classes of antimicrobial agents; and the intestinal tract of healthy poultry was a reservoir of the extended-spectrum cephalosporin-resistant isolates of serovar Infantis. (C) 2009 Elsevier B.V. All rights reserved.