'2A-Like' Signal Sequences Mediating Translational Recoding: A Novel Form of Dual Protein Targeting.
'2A-Like' Signal Sequences Mediating Translational Recoding: A Novel Form of Dual Protein Targeting.
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DOI：
10.1111/tra.12411
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
Ryan MD
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文献类型：
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作者：
Roulston C;Luke GA;de Felipe P;Ruan L;Cope J;Nicholson J;Sukhodub A;Tilsner J;Ryan MD
我们报告了N末端寡肽“2A样”序列的初步表征，该序列能够作为信号序列和翻译编码元件发挥作用。由于这种翻译重编码活性，合成了两种形式的新生多肽：（i）当2A介导的翻译重编码尚未发生时：新生多肽与2A样N末端信号序列融合，并且融合翻译产物靶向胞外途径，以及（ii）其中2A介导的翻译重编码已经发生的翻译产物：2A样信号序列作为单独的翻译产物合成，因此，新生（下游）多肽缺乏2A样信号序列并定位于细胞质。因此，这种类型的双功能信号序列导致翻译产物在两个亚细胞位点之间的分配，并代表了一种新描述的双蛋白靶向形式。
We report the initial characterization of an N‐terminal oligopeptide ‘2A‐like’ sequence that is able to function both as a signal sequence and as a translational recoding element. Owing to this translational recoding activity, two forms of nascent polypeptide are synthesized: (i) when 2A‐mediated translational recoding has not occurred: the nascent polypeptide is fused to the 2A‐like N‐terminal signal sequence and the fusion translation product is targeted to the exocytic pathway, and, (ii) a translation product where 2A‐mediated translational recoding has occurred: the 2A‐like signal sequence is synthesized as a separate translation product and, therefore, the nascent (downstream) polypeptide lacks the 2A‐like signal sequence and is localized to the cytoplasm. This type of dual‐functional signal sequence results, therefore, in the partitioning of the translation products between the two sub‐cellular sites and represents a newly described form of dual protein targeting.