THE YEAST STE12 PROTEIN BINDS TO THE DNA-SEQUENCE MEDIATING PHEROMONE INDUCTION
THE YEAST STE12 PROTEIN BINDS TO THE DNA-SEQUENCE MEDIATING PHEROMONE INDUCTION
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DOI：
10.1073/pnas.86.15.5703
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
FIELDS, S
中科院分区：
文献类型：
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作者：
DOLAN, JW;KIRKMAN, C;FIELDS, S
酿酒酵母的STE 12基因产物是转录两组细胞类型特异性基因所必需的：α-特异性基因（仅在α细胞中有活性）和α-葡萄糖依赖性基因（α-葡萄糖依赖性基因）。特异性基因（仅在α中有活性，细胞）。我们表明，放射性标记的STE 12蛋白，在体外转录和翻译制备，是能够形成复合物与未标记的DNA片段从两个特定的基因。野生型酵母，而不是ste 12突变体，产生一种因子，与来自这两个相同基因的标记DNA形成复合物。我们使用酵母提取物的测定来定位STE 12依赖性活性的结合位点。该位点对应于被鉴定为信息素诱导元件的序列，当细胞暴露于α-β时，信息素诱导元件负责基因转录的增加。因子或A因子。因此，STE 12蛋白可能是由信息素触发的信号转导途径中的最终效应物。
The STE12 gene product of Saccharomyces cerevisiae is required for the transcription of two sets of cell-type-specific genes: the a-specific genes (active only in a cells) and the .alpha.-specific genes (active only in .alpha. cells). We show that radiolabeled STE12 protein, prepared by in vitro transcription and translation, is capable of forming complexes with unlabeled DNA fragments from two a-specific genes. Wild-types yeast, but not a ste12 mutant, produce a factor that forms complexes with labeled DNA from these same two genes. We use assays with yeast extracts to localize the binding site for the STE12-dependent activity. This site corresponds to the sequence identified as the pheromone induction element, which is responsible for increased transcription of genes when cells are exposed to .alpha.-factor or a-factor. Thus the STE12 protein may be an ultimate effector in the signal transduction pathway triggered by pheromone.