A colorimetric aptasensor for the diagnosis of malaria based on cationic polymers and gold nanoparticles
A colorimetric aptasensor for the diagnosis of malaria based on cationic polymers and gold nanoparticles
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DOI：
10.1016/j.ab.2013.03.032
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发表时间：
2013-08-01
影响因子：
2.9
通讯作者：
Ban, Changill
中科院分区：
文献类型：
--
作者：
Jeon, Weejeong;Lee, Seonghwan;Ban, Changill
疟疾是由疟原虫属寄生虫引起的主要疾病负担，在热带和亚热带地区广泛传播。在这里，我们成功地开发了一种疟疾诊断技术。所提出的方法是基于疟原虫乳酸脱氢酶（pLDH），这是疟疾的生物标志物，和pL 1适体之间的相互作用，对间日疟原虫乳酸脱氢酶（PvLDH）和恶性疟原虫乳酸脱氢酶（PfLDH）。此外，阳离子聚合物，聚（二烯丙基二甲基氯化铵）（PDDA）和聚（烯丙基胺盐酸盐）（PAH），聚集金纳米颗粒（AuNP），应该可以观察到颜色从红色到蓝色的变化，这取决于pLDH的浓度。使用该适体传感器，pLDH蛋白被成功地检测到低检测限。此外，特异性测试证明，适体肽在靶向蛋白质方面比其他干扰蛋白质具有非常特异性。此外，还检测了两种主要疟疾感染者血液中的pLDH。间日疟原虫的检测限确定为PDDA为80个寄生虫/μ l，PAH为74个寄生虫/μ l。适体素具有简单、快速诊断疟疾的巨大优势。因此，开发的用于检测pLDH的适体传感器可以提供疟疾的有效和敏感的诊断。(C)2013 Elsevier Inc. All rights reserved.
Malaria, a major burden of disease caused by parasites of the genus Plasmodium, is widely spread in tropical and subtropical regions. Here, we have successfully developed a diagnostic technique for malaria. The proposed method is based on the interaction among the Plasmodium lactate dehydrogenase (pLDH), which is a biomarker for malaria, and pL1 aptamer against Plasmodium vivax lactate dehydrogenase (PvLDH) and Plasmodium falciparum lactate dehydrogenase (PfLDH). In addition, the cationic polymers, poly(diallyldimethylammonium chloride) (PDDA) and poly(allylamine hydrochloride) (PAH), aggregate gold nanoparticles (AuNPs) that should be possible to observe the change in color from red to blue, which depends on the concentration of pLDH. Using this aptasensor, pLDH proteins were successfully detected with low detection limits. Moreover, the specificity test proved that the aptasenor is very specific in targeting proteins over other interfering proteins. In addition, the pLDH from infected blood samples of the two main species of malaria were also detected. The limits of detection for P. vivax were determined as 80 parasites/mu l for PDDA and 74 parasites/mu l for PAH. The aptasenor has great advantages that can simply and rapidly diagnose malaria. Thus, the developed aptasensor for detection of pLDH can offer an effective and sensitive diagnosis of malaria. (C) 2013 Elsevier Inc. All rights reserved.