SRM targeted proteomics in search for biomarkers of HCV-induced progression of fibrosis to cirrhosis in HALT-C patients
SRM targeted proteomics in search for biomarkers of HCV-induced progression of fibrosis to cirrhosis in HALT-C patients
复制标题
DOI：
10.1002/pmic.201100601
复制
发表时间：
2012-04-01
期刊：
影响因子：
3.4
通讯作者：
Hood, Leroy
中科院分区：
文献类型：
--
作者：
Qin, Shizhen;Zhou, Yong;Hood, Leroy
目前诊断肝纤维化和肝硬化的金标准是传统的侵入性肝活检。需要用非侵入性手段评估肝纤维化。靶向蛋白质组学技术可以对蛋白质进行公正的评估，并且可能有助于鉴定与肝纤维化相关的蛋白质。我们利用选择反应监测 (SRM) 靶向蛋白质组学与器官特异性血液蛋白策略相结合来识别和量化 38 种肝脏特异性蛋白质。血清中的蛋白 C 和视黄醇结合蛋白 4 的组合作为候选生物标志物给出了有希望的初步结果，用于区分因丙型肝炎病毒 (HCV) 慢性感染而导致肝纤维化不同阶段的患者。此外，α-1-B 糖蛋白、补体因子 H 和胰岛素样生长因子结合蛋白酸不稳定亚基在区分患者和健康对照方面表现良好。
The current gold standard for diagnosis of hepatic fibrosis and cirrhosis is the traditional invasive liver biopsy. It is desirable to assess hepatic fibrosis with noninvasive means. Targeted proteomic techniques allow an unbiased assessment of proteins and might be useful to identify proteins related to hepatic fibrosis. We utilized selected reaction monitoring (SRM) targeted proteomics combined with an organ-specific blood protein strategy to identify and quantify 38 liver-specific proteins. A combination of protein C and retinol-binding protein 4 in serum gave promising preliminary results as candidate biomarkers to distinguish patients at different stages of hepatic fibrosis due to chronic infection with hepatitis C virus (HCV). Also, alpha-1-B glycoprotein, complement factor H and insulin-like growth factor binding protein acid labile subunit performed well in distinguishing patients from healthy controls.