An RNA exosome subunit mediates cell-to-cell trafficking of a homeobox mRNA via plasmodesmata
An RNA exosome subunit mediates cell-to-cell trafficking of a homeobox mRNA via plasmodesmata
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DOI：
10.1126/science.abm0840
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发表时间：
2022-01-14
期刊：
影响因子：
56.9
通讯作者：
Jackson, David
中科院分区：
文献类型：
--
作者：
Kitagawa, Munenori;Wu, Peipei;Jackson, David
信使RNA(Messenger RNAs，mRNAs)在多细胞生物体中作为细胞间通讯的移动信号。KNOTTED1(KN1)同源结构域家族转录因子通过胞间连丝在细胞间运动，控制干细胞的维持，非细胞自主作用；然而，mRNA运动的机制在很大程度上是未知的。我们发现KN1 mRNA的细胞间移动需要核糖体RNA处理蛋白44A(AtRRP44A)，这是RNA外糖体的一个亚单位，负责处理或降解真核生物中的各种RNA。AtRRP44A可以与胞间连丝相互作用，介导KN1基因的细胞间转运，遗传分析表明，AtRRP44A是拟南芥KN1同源基因--地上部MERISTEMLESS发育所必需的。我们的发现表明，AtRRP44A通过胞间连丝促进mRNA的运输，以控制植物中的干细胞依赖过程。
Messenger RNAs (mRNAs) function as mobile signals for cell-to-cell communication in multicellular organisms. The KNOTTED1 (KN1) homeodomain family transcription factors act non-cell autonomously to control stem cell maintenance in plants through cell-to-cell movement of their proteins and mRNAs through plasmodesmata; however, the mechanism of mRNA movement is largely unknown. We show that cell-to-cell movement of a KN1 mRNA requires ribosomal RNA-processing protein 44A (AtRRP44A), a subunit of the RNA exosome that processes or degrades diverse RNAs in eukaryotes. AtRRP44A can interact with plasmodesmata and mediates the cell-to-cell trafficking of KN1 mRNA, and genetic analysis indicates that AtRRP44A is required for the developmental functions of SHOOT MERISTEMLESS, an Arabidopsis KN1 homolog. Our findings suggest that AtRRP44A promotes mRNA trafficking through plasmodesmata to control stem cell-dependent processes in plants.