Nanoparticle-conjugated aptamer targeting hnRNP A2/B1 can recognize multiple tumor cells and inhibit their proliferation
Nanoparticle-conjugated aptamer targeting hnRNP A2/B1 can recognize multiple tumor cells and inhibit their proliferation
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靶向hnRNP A2/B1的纳米颗粒缀合适体可识别多种肿瘤细胞并抑制其增殖
DOI：
10.1016/j.biomaterials.2015.06.013
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发表时间：
2015-09-01
期刊：
影响因子：
14
通讯作者：
Shao, Ningsheng
中科院分区：
文献类型：
--
作者：
Li, Hui;Guo, Lei;Shao, Ningsheng
在这项研究中，我们进一步研究了先前开发的靶向ROS 17/2.8（大鼠骨肉瘤）细胞的适体。我们发现，这种C6-8适体特异性结合到异质核核糖核蛋白（hnRNP）A2/B1，它特异性标记多种肿瘤细胞系作为有效的hnRNP A2/B1单克隆抗体。当与荧光碳纳米点（CDots）结合时，它可以自由进入多种活肿瘤细胞系（HepG 2，MCF-7，H1299和HeLa），通过靶向hnRNPA 2/B1抑制其生长。当用C6-8缀合的CDots处理的GFP-HepG 2肝癌细胞植入裸鼠中时，观察到类似的抑制效果。我们的工作为靶向/标记多种肿瘤细胞类型提供了一种新的适体，其纳米颗粒缀合物带来了进一步的优势，增加了其在癌症诊断和治疗中的应用潜力。(C)2015爱思唯尔有限公司版权所有。
In this study, we further investigated a previously developed aptamer targeting ROS 17/2.8 (rat osteosarcoma) cells. We found that this C6-8 aptamer specifically binds to heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 and that it specifically labeled multiple tumor-cell lines as effectively as hnRNP A2/B1 monoclonal antibodies. When conjugated with fluorescent carbon nanodots (CDots) it could freely enter multiple living tumor cell lines (HepG2, MCF-7, H1299, and HeLa), whose growth it inhibited by targeting hnRNP A2/B1. Similar inhibitory effects were observed when the GFP-HepG2 hepatocarcinoma cells treated with C6-8-conjugated CDots were implanted in nude mice. Our work provides a new aptamer for targeting/labeling multiple tumor cell types, and its nanoparticle conjugates bring further advantages that increase its potential for use in cancer diagnosis and therapy. (C) 2015 Elsevier Ltd. All rights reserved.