Significant fetal-maternal hemorrhage after termination of pregnancy: Implications for development of fetal cell microchimerism
Significant fetal-maternal hemorrhage after termination of pregnancy: Implications for development of fetal cell microchimerism
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DOI：
10.1067/mob.2001.111072
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发表时间：
2001-03-01
影响因子：
9.8
通讯作者：
Klinger, KW
中科院分区：
文献类型：
--
作者：
Bianchi, DW;Farina, A;Klinger, KW
目的：最近有报道称胎儿细胞微嵌合与自身免疫性疾病之间存在关联，这增加了人们对胎儿细胞产后持久性的兴趣。本研究的目的是通过定量聚合酶链反应扩增来确定择期终止妊娠后是否发生明显的胎母出血。研究设计：从23名在静脉穿刺前立即终止妊娠的妇女中采集血液样本；用y染色体引物进行定量聚合酶链反应扩增。其中男胎21例，女胎2例。将结果平衡为16 mL，并通过加权线性回归分析来评估检测到的胎儿有核细胞当量与妊娠周数之间的相关性。结果：在已知的21例男性胎儿中，检测到的胎儿有核细胞当量的中位数为1552（范围50-37,618）。雌性胎儿没有检测到胎儿有核细胞等价物。男性胎儿有核细胞当量与胎龄呈正相关(P
OBJECTIVE: Recent reports that an association exists between fetal cell microchimerism and autoimmune disease has increased interest in the postpartum persistence of fetal cells. The purpose of this study was to determine, by means of quantitative polymerase chain reaction amplification, whether a significant fetal-maternal hemorrhage occurs after elective termination of pregnancy.STUDY DESIGN: Blood samples were obtained from 23 women who underwent termination of pregnancy immediately before venipuncture; these samples were subjected to analysis by quantitative polymerase chain reaction amplification with the use of Y-chromosome primers. There were 21 male and 2 female fetuses. Results were equilibrated to 16 mL and analyzed by a weighted linear regression analysis to evaluate the correlation between detected fetal nucleated cell equivalents and gestational weeks.RESULTS: Among the 21 known male fetuses, the median number of detected fetal nucleated cell equivalents was 1552 (range, 50-37,618). The female fetuses had no fetal nucleated cell equivalents detected. A positive dependence of male fetal nucleated cell equivalents on gestational age was shown (P