In vitro and in vivo growth inhibition of drug-resistant ovarian carcinoma cells using a combination of cisplatin and a TRAIL-encoding retrovirus.
In vitro and in vivo growth inhibition of drug-resistant ovarian carcinoma cells using a combination of cisplatin and a TRAIL-encoding retrovirus.
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使用顺铂和编码 TRAIL 的逆转录病毒的组合在体外和体内抑制耐药卵巢癌细胞的生长。
DOI：
10.3892/ol.2012.926
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发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
X. Wen
中科院分区：
文献类型：
--
作者：
Fang Li;Yi Guo;Ling;Yankang Duan;Fang Fang;S. Niu;Qiujie Ba;Huaishi Zhu;Fanfei Kong;Chao Lin;X. Wen
通过使用编码肿瘤坏死因子相关凋亡诱导配体（TRAIL）的质粒瞬时转染逆转录病毒包装细胞系BOSC 23，产生编码TNF相关凋亡诱导配体（TRAIL）基因的逆转录病毒。逆转录病毒能在体外杀伤耐药的A2780/DDP卵巢癌细胞，并诱导细胞表达TRAIL。此外，TRAIL蛋白通过半胱天冬酶激活的凋亡机制导致细胞的生长抑制。已证实，将此类细胞暴露于顺铂与编码TRAIL的逆转录病毒的组合导致体外和裸鼠模型中的异种移植物A2780/DDP肿瘤中更高的抗癌活性。本研究提示化疗联合TRAIL基因治疗可能是治疗耐药卵巢癌的一种有效方法。
Retroviruses encoding the TNF-related apoptosis-inducing ligand (TRAIL) gene were generated by transient transfection of the retrovirus packing cell line BOSC 23 using TRAIL-encoding plasmid. The retrovirus was able to transduce drug-resistant A2780/DDP ovarian carcinoma cells in vitro and induce TRAIL expression in the cells, as detected by western blot assay. Furthermore, the TRAIL protein led to the growth inhibition of the cells via a caspase-activated apoptotic mechanism. It was confirmed that exposure of such cells to cisplatin in combination with the TRAIL-encoding retrovirus resulted in higher anticancer activity in vitro and in the xenograft A2780/DDP tumor in a nude mouse model. This study suggests that chemotherapy in combination with TRAIL gene therapy may be an efficient approach to treat drug-resistant ovarian cancer.
DOI：
10.1073/pnas.90.18.8392
发表时间：
1993-09-15
影响因子：
11.1
作者：
PEAR, WS;NOLAN, GP;BALTIMORE, D
通讯作者：
BALTIMORE, D