Characterization of MADS-box genes in charophycean green algae and its implication for the evolution of MADS-box genes.
Characterization of MADS-box genes in charophycean green algae and its implication for the evolution of MADS-box genes.
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DOI：
10.1073/pnas.0409860102
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发表时间：
2005-02
影响因子：
11.1
通讯作者：
Y. Tanabe;M. Hasebe;H. Sekimoto;T. Nishiyama;M. Kitani;K. Henschel;Thomas Münster;G. Theißen;H. Nozaki;M. Ito
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文献类型：
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作者：
Y. Tanabe;M. Hasebe;H. Sekimoto;T. Nishiyama;M. Kitani;K. Henschel;Thomas Münster;G. Theißen;H. Nozaki;M. Ito
陆地植物的MADS盒基因广泛分化形成一个超家族，在发育的各个方面都很重要，包括花器官的特异性作为同源异型选择基因。陆地植物的近亲是淡水绿色藻类轮藻。为了研究陆生植物MADS盒基因的起源和进化，我们在三种轮藻科绿色藻类中鉴定了这些基因：石藻Chara globularis，鞘毛菌Coleochaete scutata，和desmid新月藻peracerosum-strigosum-littorale复合体。系统发育分析表明，MADS-box基因广泛分歧的陆生植物谱系轮藻分离后，从陆生植物。石藻C.在卵原细胞和精子器分化过程中，球状体mRNA与卵和精子一起被特异性地检测到。C. peracerosum-strigosum-littorale-complex基因在营养细胞开始分化为配子囊细胞时增加，受精后减少。这些表达模式表明，陆地植物MADS-box基因的前体最初在单倍体生殖细胞分化中起作用，并且在陆地植物的进化过程中，单倍体MADS-box基因被招募到二倍体世代中。
The MADS-box genes of land plants are extensively diverged to form a superfamily and are important in various aspects of development including the specification of floral organs as homeotic selector genes. The closest relatives of land plants are the freshwater green algae charophyceans. To study the origin and evolution of land plant MADS-box genes, we characterized these genes in three charophycean green algae: the stonewort Chara globularis, the coleochaete Coleochaete scutata, and the desmid Closterium peracerosum-strigosum-littorale complex. Phylogenetic analyses suggested that MADS-box genes diverged extensively in the land plant lineage after the separation of charophyceans from land plants. The stonewort C. globularis mRNA was specifically detected in the oogonium and antheridium together with the egg and spermatozoid during their differentiation. The expression of the C. peracerosum-strigosum-littorale-complex gene increased when vegetative cells began to differentiate into gametangial cells and decreased after fertilization. These expression patterns suggest that the precursors of land plant MADS-box genes originally functioned in haploid reproductive cell differentiation and that the haploid MADS-box genes were recruited into a diploid generation during the evolution of land plants.