Salicylic acid-related cotton (Gossypium arboreum) ribosomal protein GaRPL18 contributes to resistance to Verticillium dahliae.
Salicylic acid-related cotton (Gossypium arboreum) ribosomal protein GaRPL18 contributes to resistance to Verticillium dahliae.
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DOI：
10.1186/s12870-017-1007-5
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发表时间：
2017-03-03
影响因子：
5.3
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Gong Q;Yang Z;Wang X;Butt HI;Chen E;He S;Zhang C;Zhang X;Li F
大丽花黄萎病（Verticillium dahliae）是一种引起血管性枯萎病的植物致病性真菌病原体，导致棉花产量大幅下降。棉花抗黄萎病的复杂机制尚不清楚。鉴定一种内源性抗性基因可能有助于控制此病。我们克隆了抗黄萎病棉花（Gossypium arboreum）的核糖体蛋白L18 （GaRPL18）基因，该基因介导对黄萎病的抗性。然后，我们对该基因在棉花和拟南芥植物中的功能进行了表征。GaRPL18编码60S核糖体蛋白亚基，对细胞内蛋白的生物合成很重要。然而，先前的研究表明，一些核糖体蛋白对人类的肿瘤发生和先天性疾病也有抑制作用，并在植物病害防御中发挥作用。在这里，我们观察到大丽花弧菌感染诱导GaRPL18的表达。此外，我们确定了GaRPL18的表达模式与不同棉花品种的抗病水平是一致的。在水杨酸（SA）处理下，GaRPL18的表达上调，提示GaRPL18参与了SA信号转导通路。采用病毒诱导基因沉默技术测定GaRPL18表达水平是否影响棉花抗病性。由于免疫相关分子丰度的显著降低，GaRPL18被沉默的抗枯萎棉花品种比对照植物更容易受到大丽花病菌的感染。采用花浸法对拟南芥生态型Columbia （Col-0）植株进行了GaRPL18转化。过表达GaRPL18的植株比野生型Col-0植株对大丽花弧菌的抗性更强。转基因拟南芥对大丽花的抗性增强可能是通过SA途径介导的。我们的研究结果为GaRPL18的作用提供了新的见解，表明GaRPL18在棉花“癌症”（也称为黄萎病）的抗性中起着至关重要的作用，主要由sa相关的信号通路机制调节。本文的在线版本（doi:10.1186/s12870-017-1007-5）包含补充材料，仅供授权用户使用。
Verticillium dahliae is a phytopathogenic fungal pathogen that causes vascular wilt diseases responsible for considerable decreases in cotton yields. The complex mechanism underlying cotton resistance to Verticillium wilt remains uncharacterized. Identifying an endogenous resistance gene may be useful for controlling this disease. We cloned the ribosomal protein L18 (GaRPL18) gene, which mediates resistance to Verticillium wilt, from a wilt-resistant cotton species (Gossypium arboreum). We then characterized the function of this gene in cotton and Arabidopsis thaliana plants. GaRPL18 encodes a 60S ribosomal protein subunit important for intracellular protein biosynthesis. However, previous studies revealed that some ribosomal proteins are also inhibitory toward oncogenesis and congenital diseases in humans and play a role in plant disease defense. Here, we observed that V. dahliae infections induce GaRPL18 expression. Furthermore, we determined that the GaRPL18 expression pattern is consistent with the disease resistance level of different cotton varieties. GaRPL18 expression is upregulated by salicylic acid (SA) treatments, suggesting the involvement of GaRPL18 in the SA signal transduction pathway. Virus-induced gene silencing technology was used to determine whether the GaRPL18 expression level influences cotton disease resistance. Wilt-resistant cotton species in which GaRPL18 was silenced became more susceptible to V. dahliae than the control plants because of a significant decrease in the abundance of immune-related molecules. We also transformed A. thaliana ecotype Columbia (Col-0) plants with GaRPL18 according to the floral dip method. The plants overexpressing GaRPL18 were more resistant to V. dahliae infections than the wild-type Col-0 plants. The enhanced resistance of transgenic A. thaliana plants to V. dahliae is likely mediated by the SA pathway. Our findings provide new insights into the role of GaRPL18, indicating that it plays a crucial role in resistance to cotton “cancer”, also known as Verticillium wilt, mainly regulated by an SA-related signaling pathway mechanism. The online version of this article (doi:10.1186/s12870-017-1007-5) contains supplementary material, which is available to authorized users.