A role for the yeast SWI/SNF complex in DNA replication
A role for the yeast SWI/SNF complex in DNA replication
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DOI：
10.1093/nar/27.9.2022
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发表时间：
1999-05-01
影响因子：
14.9
通讯作者：
Peterson, CL
中科院分区：
文献类型：
--
作者：
Flanagan, JF;Peterson, CL
酵母SWI/SNF复合体是许多基因表达所必需的，也是几种转录激活剂充分发挥功能所必需的。遗传学和生物化学研究表明，SWI/SNF利用ATP水解的能量来对抗染色质介导的转录抑制。我们已经测试了SWI/SNF可能也在DNA复制中发挥作用的可能性。有丝分裂微染色体稳定性分析被用来研究不同自主复制序列(ARSS)在细胞中的复制效率。存在和不存在SWI/SNF。含有ARS1、ARS309或ARS307的微小染色体的稳定性不会因缺乏SWI/SNF而改变，而当SWI/SNF失活时，ARS121的功能会受到削弱。ARS121的SWI/SNF依赖性不需要复制：增强子因子ABF1，因此，它似乎是ARS121最小起源的属性。同样，缺乏ABF1复制增强子的ARS1的最小衍生物获得对SWI/SNF的依赖。用LexA-GAL4嵌合激活子的结合位点取代ARS1上的ABF1结合位点也创建了依赖SWI/SNF的ARS。我们的研究表明，SWI/SNF染色质重塑复合体可以在BASH复制和转录中发挥作用；此外，ARS元件对SWI/SNF的依赖既是ARS特异性复制增强子的特性，也是ARS序列元件整体组织的特性。
The yeast SWI/SNF complex is required for expression of many genes and for the-full functioning of several transcriptional activators. Genetic and biochemical studies indicate that SWI/SNF uses the energy of ATP hydrolysis to antagonize chromatin-mediated transcriptional repression. We have tested the possibility that SWI/SNF might also play a role in DNA replication. A mitotic minichromosome stability assay was used to investigate the replication efficiency of a variety of autonomous replication sequences (ARSs) in the. presence and absence of SWI/SNF. The stability of minichromosomes that contain ARS1, ARS309 or ARS307 is not altered by lack of SWI/SNF, whereas the functioning of ARS121 is crippled when SWI/SNF is inactivated. The SWI/SNF dependence of ARS121 does not require the replication:enhancer factor, ABF1, and thus, it appears to be a property of a minimal ARS121 origin. Likewise, a minimal derivative of ARS1 that lacks the ABF1 replication enhancer acquires SWI/SNF dependence. Replacing the ABF1 binding site at ARS1 with a binding site for the LexA-GAL4 chimeric activator also creates a SWI/SNF-dependent ARS. Our studies suggest that the SWI/SNF chromatin remodeling complex can play a role in bath replication and transcription and, furthermore; that SWI/SNF dependence of ARS elements is a property of both an ARS-specific replication enhancer and the overall organization of ARS sequence elements.