Melatonin Increases Bladder Capacity via GABAergic System and Decreases Urine Volume in Rats
Melatonin Increases Bladder Capacity via GABAergic System and Decreases Urine Volume in Rats
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DOI：
10.1016/j.juro.2010.03.002
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发表时间：
2010-07-01
影响因子：
6.6
通讯作者：
Yokoyama, Osamu
中科院分区：
文献类型：
--
作者：
Matsuta, Yosuke;Yusup, Anwar;Yokoyama, Osamu
目的：褪黑激素产生受损会扰乱正常的昼夜睡眠模式，从而导致老年人夜尿。褪黑激素改善食欲。我们推测褪黑激素可能通过抑制大脑排尿中枢来减轻尿频。材料和方法：在清醒的雌性Sprague-Dawley（R）大鼠（日本SLC，Hamamatsu，日本）中进行膀胱测压。我们检测了单独使用褪黑激素（脑室内注射4.3 × 10（-1）至4.3 × 10 pmol）或与γ-氨基丁酸（A）拮抗剂荷包牡丹碱（静脉注射5.0 × 10（-5）mg/kg）对膀胱功能的影响。在水负荷大鼠中研究褪黑激素（4.3 × 10 pmol脑室内）对尿量的影响。在4.3 x 10-1，4.3和4.3 x 10 pmol时，褪黑素剂量依赖性地分别增加膀胱容量27.0%，40.8%和63.5%，但对膀胱收缩压无显著影响。荷包牡丹碱抑制褪黑激素诱导的膀胱容量增加。褪黑激素减少尿量在水负荷大鼠，但血浆抗利尿激素，心房钠尿肽和膀胱收缩pressure were not changed.Conclusions：结果表明，褪黑激素增加膀胱容量通过γ-氨基丁酸（A）受体在大脑和减少尿量。因此，褪黑激素可以通过中枢神经系统作用有益于糖尿病。
Purpose: Impaired melatonin production is involved in disruption of the normal circadian pattern of sleep, which leads to nocturia in older adults. Melatonin improves nocturia. We hypothesized that melatonin could alleviate urinary frequency by suppressing the brain micturition center. We investigated the central contribution of melatonin to bladder function and urine volume.Materials and Methods: Cystometry was done in conscious female Sprague-Dawley (R) rats (Japan SLC, Hamamatsu, Japan). We examined the effect of melatonin alone (4.3 x 10(-1) to 4.3 x 10 pmol intracerebroventricularly) or with the gamma-aminobutyric acid(A) antagonist bicuculline (5.0 x 10(-5) mg/kg intravenously) on bladder function. The influence of melatonin (4.3 x 10 pmol intracerebroventricularly) on urine volume was investigated in water loaded rats. Blood samples were collected to determine antidiuretic hormone, atrial natriuretic peptide and brain natriuretic peptide 4 hours after melatonin administration.Results: Melatonin significantly increased bladder capacity dose dependently by 27.0%, 40.8% and 63.5% at 4.3 x 10-1, 4.3 and 4.3 x 10 pmol, respectively, but had no significant effect on bladder contraction pressure. Bicuculline inhibited the melatonin induced increases in bladder capacity. Melatonin decreased urine volume in water loaded rats but plasma antidiuretic hormone, atrial natriuretic peptide and bladder contraction pressure were not changed.Conclusions: Results suggest that melatonin increases bladder capacity via gamma-aminobutyric acid(A) receptor in the brain and decreases urine volume. Thus, melatonin could be beneficial for nocturia via a central nervous system effect.