Salivary markers of oxidative stress and periodontal pathogens in patients with periodontitis from Santander, Colombia
Salivary markers of oxidative stress and periodontal pathogens in patients with periodontitis from Santander, Colombia
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DOI：
10.7705/biomedica.5149
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发表时间：
2020-05-01
期刊：
影响因子：
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通讯作者：
Alfonso, Pedro A.
中科院分区：
文献类型：
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作者：
Sánchez-Villamil, Juana P.;Pino-Vélez, Carolina;Alfonso, Pedro A.
导言：牙周炎影响着超过20%的拉丁美洲人口。氧化标记物与牙周炎的发生发展密切相关，因此，其在牙周炎发病机制中的作用有待进一步研究。目的：了解牙周炎患者唾液中主要细菌和病毒的流行情况，评估患者唾液中的总抗氧化能力和脂质过氧化水平。材料和方法：根据美国疾病控制与预防中心和美国牙周学学会的诊断标准，对101例健康受试者进行了系统的横断面研究，其中87例被诊断为牙周炎(P)，14例无牙周袋作为对照(C)。用聚合酶链式反应技术对龈下标本中的主要病毒和牙科病原菌进行鉴定。结果：牙周炎患者牙周深度袋平均值为5.6±1.7 mm，临床附着丧失平均值为6.1±3.1 mm。最常见的微生物是伴生放线杆菌(32.5%)和牙龈卟啉单胞菌(18.6%)。来自农村的患者伴生放线菌比例较高(城市：17.9%，农村：48.9%，p=0.0018)。在牙周炎患者中，EBV、HSV1、HSV2和HCMV基因的频率为2.3%。牙周炎患者血清丙二醛水平(P：2.1±1.5；C：0.46±0.3摩尔/g蛋白；P=0.0001)和总抗氧化能力(P：0.32±0.2；C：0.15±0.1 mm；P<；0.0036)明显高于对照组。结论：伴生放线杆菌是牙周炎患者唾液中最常见的细菌，其存在并不改变牙周炎患者唾液中氧化标志物的水平。
INTRODUCTION: Periodontitis affects more than 20% of the Latin American population. Oxidative markers are associated with greater progression of periodontitis; therefore, its role in pathogenesis should be studied.OBJECTIVE: To determine the prevalence of the main oral bacteria and viruses associated with periodontitis and estimate the total antioxidant capacity and lipid peroxidation in saliva from patients with periodontitis.MATERIALS AND METHODS: We conducted systemically a cross-sectional study in 101 healthy subjects, 87 of whom had been diagnosed with periodontitis (P), according to the criteria of the Centers of Disease Control and Prevention and the American Academy of Periodontology, and 14 without periodontal pockets as controls (C). In subgingival samples, major viruses and dental pathogenic bacteria were identified using PCR techniques. The levels of total antioxidant capacity and malon-di-aldehyde (MDA) were determined by spectrophotometry in samples of unstimulated saliva.RESULTS: The mean of periodontal depth pocket and clinical attachment loss in patients with periodontitis was 5.6 ± 1.7 and 6.1 ± 3.1 mm, respectively. The most prevalent microorganisms were Aggregatibacter actinomycetemcomitans (32.5%) and Porphyromonas gingivalis (18.6%). The patients from rural areas showed a higher percentage of A. actinomycetemcomitans (urban: 17.9% vs. rural: 48.9%, p=0.0018). In patients with periodontitis, the frequency of EBV, HSV1 and 2, and HCMV genes was 2.3%. Periodontitis patients had higher levels of MDA (P: 2.1 ± 1.5; C: 0.46 ± 0.3 mumol/g protein; p=0.0001) and total antioxidant capacity (P: 0.32 ± 0.2; C: 0.15 ± 0.1 mM; p< 0.0036). Oxidative markers showed no modifications due to the presence of periodontopathic bacteria.CONCLUSIONS: Aggregatibacter actinomycetemcomitans was the most prevalent bacteria; its presence did not modify the levels of oxidative markers in the saliva of patients with periodontitis.